iGUIDE: an improved pipeline for analyzing CRISPR cleavage specificity

Genome engineering methods have advanced greatly with the development of programmable nucleases, but methods for quantifying on- and off-target cleavage sites and associated deletions remain nascent. Here, we report an improvement of the GUIDE-seq method, iGUIDE, which allows filtering of mispriming...

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Detalles Bibliográficos
Autores principales: Nobles, Christopher L., Reddy, Shantan, Salas-McKee, January, Liu, Xiaojun, June, Carl H., Melenhorst, J. Joseph, Davis, Megan M., Zhao, Yangbing, Bushman, Frederic D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337799/
https://www.ncbi.nlm.nih.gov/pubmed/30654827
http://dx.doi.org/10.1186/s13059-019-1625-3

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