Cargando…
The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation
BACKGROUND: Worldwide, the increasing use of antibiotics has resulted in antimicrobial resistance, leading to studies to find alternative antimicrobial treatments. Tea polyphenols have antibacterial properties. Bacteriocins produced by probiotic lactobacilli can inhibit Gram-positive bacteria. This...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338016/ https://www.ncbi.nlm.nih.gov/pubmed/30670678 http://dx.doi.org/10.12659/MSM.912971 |
_version_ | 1783388384502218752 |
---|---|
author | Xu, Zhonghua Li, Jin Zhou, Xindie Dai, Jianbo Zhang, Junjie Huang, Yong Xu, Nanwei |
author_facet | Xu, Zhonghua Li, Jin Zhou, Xindie Dai, Jianbo Zhang, Junjie Huang, Yong Xu, Nanwei |
author_sort | Xu, Zhonghua |
collection | PubMed |
description | BACKGROUND: Worldwide, the increasing use of antibiotics has resulted in antimicrobial resistance, leading to studies to find alternative antimicrobial treatments. Tea polyphenols have antibacterial properties. Bacteriocins produced by probiotic lactobacilli can inhibit Gram-positive bacteria. This study used a rabbit model of infection, following femoral fracture with internal fixation, to evaluate the efficacy of the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin. MATERIAL/METHODS: Twenty-four New Zealand White rabbits underwent femoral fracture, internal fixation, and insertion of a mini-titanium implant, and were inoculated intravenously with suspensions of Staphylococcal bacteria. Four treatment groups included group A, injected with tea polyphenols and bacteriocins (N=6); group B, injected with cefradine and bacteriocins (N=6); group C, injected with tea polyphenols and cefradine (N=6); and group D (controls), injected with saline (N=6). Blood samples were collected at 1, 6, 12, 24, and 48 hours after the injection of bacteriocins. Biofilms that formed on the mini-titanium implant were studied by fluorescence microscopy. Serum levels of level of interleukin (IL)-8, IL-6, and tumor necrosis factor-α (TNF-α) were measured using an enzyme-linked immunosorbent assay (ELISA). RESULTS: The combination of tea polyphenols and bacteriocins (group A) had a significant inhibitory effect on Staphylococcus aureus (P<0.05) and significant differences in serum levels of IL-8, TNF-α, and IL-6 levels in serum (P<0.05) when compared with groups, B, C, and D. CONCLUSIONS: In a rabbit model of femoral fracture with internal fixation, the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin effectively controlled Staphylococcus aureus infection. |
format | Online Article Text |
id | pubmed-6338016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63380162019-01-29 The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation Xu, Zhonghua Li, Jin Zhou, Xindie Dai, Jianbo Zhang, Junjie Huang, Yong Xu, Nanwei Med Sci Monit Animal Study BACKGROUND: Worldwide, the increasing use of antibiotics has resulted in antimicrobial resistance, leading to studies to find alternative antimicrobial treatments. Tea polyphenols have antibacterial properties. Bacteriocins produced by probiotic lactobacilli can inhibit Gram-positive bacteria. This study used a rabbit model of infection, following femoral fracture with internal fixation, to evaluate the efficacy of the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin. MATERIAL/METHODS: Twenty-four New Zealand White rabbits underwent femoral fracture, internal fixation, and insertion of a mini-titanium implant, and were inoculated intravenously with suspensions of Staphylococcal bacteria. Four treatment groups included group A, injected with tea polyphenols and bacteriocins (N=6); group B, injected with cefradine and bacteriocins (N=6); group C, injected with tea polyphenols and cefradine (N=6); and group D (controls), injected with saline (N=6). Blood samples were collected at 1, 6, 12, 24, and 48 hours after the injection of bacteriocins. Biofilms that formed on the mini-titanium implant were studied by fluorescence microscopy. Serum levels of level of interleukin (IL)-8, IL-6, and tumor necrosis factor-α (TNF-α) were measured using an enzyme-linked immunosorbent assay (ELISA). RESULTS: The combination of tea polyphenols and bacteriocins (group A) had a significant inhibitory effect on Staphylococcus aureus (P<0.05) and significant differences in serum levels of IL-8, TNF-α, and IL-6 levels in serum (P<0.05) when compared with groups, B, C, and D. CONCLUSIONS: In a rabbit model of femoral fracture with internal fixation, the combined use of tea polyphenols and Lactobacillus plantarum ST8SH bacteriocin effectively controlled Staphylococcus aureus infection. International Scientific Literature, Inc. 2019-01-10 /pmc/articles/PMC6338016/ /pubmed/30670678 http://dx.doi.org/10.12659/MSM.912971 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Xu, Zhonghua Li, Jin Zhou, Xindie Dai, Jianbo Zhang, Junjie Huang, Yong Xu, Nanwei The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title | The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title_full | The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title_fullStr | The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title_full_unstemmed | The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title_short | The Combined Use of Tea Polyphenols and Lactobacillus Plantarum ST8SH Bacteriocin in a Rabbit Model of Infection Following Femoral Fracture with Internal Fixation |
title_sort | combined use of tea polyphenols and lactobacillus plantarum st8sh bacteriocin in a rabbit model of infection following femoral fracture with internal fixation |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338016/ https://www.ncbi.nlm.nih.gov/pubmed/30670678 http://dx.doi.org/10.12659/MSM.912971 |
work_keys_str_mv | AT xuzhonghua thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT lijin thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT zhouxindie thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT daijianbo thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT zhangjunjie thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT huangyong thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT xunanwei thecombineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT xuzhonghua combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT lijin combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT zhouxindie combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT daijianbo combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT zhangjunjie combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT huangyong combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation AT xunanwei combineduseofteapolyphenolsandlactobacillusplantarumst8shbacteriocininarabbitmodelofinfectionfollowingfemoralfracturewithinternalfixation |