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Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain
The relationships between cerebrospinal fluid (CSF) and brain interstitial fluid are still being elucidated. It has been proposed that CSF within the subarachnoid space will enter paravascular spaces along arteries to flush through the parenchyma of the brain. However, CSF also directly exits the su...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338719/ https://www.ncbi.nlm.nih.gov/pubmed/30306266 http://dx.doi.org/10.1007/s00401-018-1916-x |
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author | Ma, Qiaoli Ries, Miriam Decker, Yann Müller, Andreas Riner, Chantal Bücker, Arno Fassbender, Klaus Detmar, Michael Proulx, Steven T. |
author_facet | Ma, Qiaoli Ries, Miriam Decker, Yann Müller, Andreas Riner, Chantal Bücker, Arno Fassbender, Klaus Detmar, Michael Proulx, Steven T. |
author_sort | Ma, Qiaoli |
collection | PubMed |
description | The relationships between cerebrospinal fluid (CSF) and brain interstitial fluid are still being elucidated. It has been proposed that CSF within the subarachnoid space will enter paravascular spaces along arteries to flush through the parenchyma of the brain. However, CSF also directly exits the subarachnoid space through the cribriform plate and other perineural routes to reach the lymphatic system. In this study, we aimed to elucidate the functional relationship between CSF efflux through lymphatics and the potential influx into the brain by assessment of the distribution of CSF-infused tracers in awake and anesthetized mice. Using near-infrared fluorescence imaging, we showed that tracers quickly exited the subarachnoid space by transport through the lymphatic system to the systemic circulation in awake mice, significantly limiting their spread to the paravascular spaces of the brain. Magnetic resonance imaging and fluorescence microscopy through the skull under anesthetized conditions indicated that tracers remained confined to paravascular spaces on the surface of the brain. Immediately after death, a substantial influx of tracers occurred along paravascular spaces extending into the brain parenchyma. We conclude that under normal conditions a rapid CSF turnover through lymphatics precludes significant bulk flow into the brain. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00401-018-1916-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6338719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-63387192019-02-01 Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain Ma, Qiaoli Ries, Miriam Decker, Yann Müller, Andreas Riner, Chantal Bücker, Arno Fassbender, Klaus Detmar, Michael Proulx, Steven T. Acta Neuropathol Original Paper The relationships between cerebrospinal fluid (CSF) and brain interstitial fluid are still being elucidated. It has been proposed that CSF within the subarachnoid space will enter paravascular spaces along arteries to flush through the parenchyma of the brain. However, CSF also directly exits the subarachnoid space through the cribriform plate and other perineural routes to reach the lymphatic system. In this study, we aimed to elucidate the functional relationship between CSF efflux through lymphatics and the potential influx into the brain by assessment of the distribution of CSF-infused tracers in awake and anesthetized mice. Using near-infrared fluorescence imaging, we showed that tracers quickly exited the subarachnoid space by transport through the lymphatic system to the systemic circulation in awake mice, significantly limiting their spread to the paravascular spaces of the brain. Magnetic resonance imaging and fluorescence microscopy through the skull under anesthetized conditions indicated that tracers remained confined to paravascular spaces on the surface of the brain. Immediately after death, a substantial influx of tracers occurred along paravascular spaces extending into the brain parenchyma. We conclude that under normal conditions a rapid CSF turnover through lymphatics precludes significant bulk flow into the brain. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00401-018-1916-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-10-10 2019 /pmc/articles/PMC6338719/ /pubmed/30306266 http://dx.doi.org/10.1007/s00401-018-1916-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Ma, Qiaoli Ries, Miriam Decker, Yann Müller, Andreas Riner, Chantal Bücker, Arno Fassbender, Klaus Detmar, Michael Proulx, Steven T. Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title | Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title_full | Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title_fullStr | Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title_full_unstemmed | Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title_short | Rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
title_sort | rapid lymphatic efflux limits cerebrospinal fluid flow to the brain |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338719/ https://www.ncbi.nlm.nih.gov/pubmed/30306266 http://dx.doi.org/10.1007/s00401-018-1916-x |
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