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Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing

Rapid delivery of proper antibiotic therapies to infectious disease patients is essential for improving patient outcomes, decreasing hospital lengths-of-stay, and combating the antibiotic resistance epidemic. Antibiotic stewardship programs are designed to address these issues by coordinating hospit...

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Autores principales: Flentie, Kelly, Spears, Benjamin R, Chen, Felicia, Purmort, Nathan B, DaPonte, Kayla, Viveiros, Emma, Phelan, Nicholas, Krebill, Cicely, Flyer, Alec N., Hooper, David C, Smalley, David L., Ferraro, Mary Jane, Vacic, Aleksandar, Stern, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338723/
https://www.ncbi.nlm.nih.gov/pubmed/30659207
http://dx.doi.org/10.1038/s41598-018-35916-0
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author Flentie, Kelly
Spears, Benjamin R
Chen, Felicia
Purmort, Nathan B
DaPonte, Kayla
Viveiros, Emma
Phelan, Nicholas
Krebill, Cicely
Flyer, Alec N.
Hooper, David C
Smalley, David L.
Ferraro, Mary Jane
Vacic, Aleksandar
Stern, Eric
author_facet Flentie, Kelly
Spears, Benjamin R
Chen, Felicia
Purmort, Nathan B
DaPonte, Kayla
Viveiros, Emma
Phelan, Nicholas
Krebill, Cicely
Flyer, Alec N.
Hooper, David C
Smalley, David L.
Ferraro, Mary Jane
Vacic, Aleksandar
Stern, Eric
author_sort Flentie, Kelly
collection PubMed
description Rapid delivery of proper antibiotic therapies to infectious disease patients is essential for improving patient outcomes, decreasing hospital lengths-of-stay, and combating the antibiotic resistance epidemic. Antibiotic stewardship programs are designed to address these issues by coordinating hospital efforts to rapidly deliver the most effective antibiotics for each patient, which requires bacterial identification and antimicrobial susceptibility testing (AST). Despite the clinical need for fast susceptibility testing over a wide range of antibiotics, conventional phenotypic AST requires overnight incubations, and new rapid phenotypic AST platforms restrict the number of antibiotics tested for each patient. Here, we introduce a novel approach to AST based on signal amplification of bacterial surfaces that enables phenotypic AST within 5 hours for non-fastidious bacteria. By binding bacterial surfaces, this novel method allows more accurate measurements of bacterial replication in instances where organisms filament or swell in response to antibiotic exposure. Further, as an endpoint assay performed on standard microplates, this method should enable parallel testing of more antibiotics than is currently possible with available automated systems. This technology has the potential to revolutionize clinical practice by providing rapid and accurate phenotypic AST data for virtually all available antibiotics in a single test.
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spelling pubmed-63387232019-01-22 Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing Flentie, Kelly Spears, Benjamin R Chen, Felicia Purmort, Nathan B DaPonte, Kayla Viveiros, Emma Phelan, Nicholas Krebill, Cicely Flyer, Alec N. Hooper, David C Smalley, David L. Ferraro, Mary Jane Vacic, Aleksandar Stern, Eric Sci Rep Article Rapid delivery of proper antibiotic therapies to infectious disease patients is essential for improving patient outcomes, decreasing hospital lengths-of-stay, and combating the antibiotic resistance epidemic. Antibiotic stewardship programs are designed to address these issues by coordinating hospital efforts to rapidly deliver the most effective antibiotics for each patient, which requires bacterial identification and antimicrobial susceptibility testing (AST). Despite the clinical need for fast susceptibility testing over a wide range of antibiotics, conventional phenotypic AST requires overnight incubations, and new rapid phenotypic AST platforms restrict the number of antibiotics tested for each patient. Here, we introduce a novel approach to AST based on signal amplification of bacterial surfaces that enables phenotypic AST within 5 hours for non-fastidious bacteria. By binding bacterial surfaces, this novel method allows more accurate measurements of bacterial replication in instances where organisms filament or swell in response to antibiotic exposure. Further, as an endpoint assay performed on standard microplates, this method should enable parallel testing of more antibiotics than is currently possible with available automated systems. This technology has the potential to revolutionize clinical practice by providing rapid and accurate phenotypic AST data for virtually all available antibiotics in a single test. Nature Publishing Group UK 2019-01-18 /pmc/articles/PMC6338723/ /pubmed/30659207 http://dx.doi.org/10.1038/s41598-018-35916-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Flentie, Kelly
Spears, Benjamin R
Chen, Felicia
Purmort, Nathan B
DaPonte, Kayla
Viveiros, Emma
Phelan, Nicholas
Krebill, Cicely
Flyer, Alec N.
Hooper, David C
Smalley, David L.
Ferraro, Mary Jane
Vacic, Aleksandar
Stern, Eric
Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title_full Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title_fullStr Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title_full_unstemmed Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title_short Microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
title_sort microplate-based surface area assay for rapid phenotypic antibiotic susceptibility testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338723/
https://www.ncbi.nlm.nih.gov/pubmed/30659207
http://dx.doi.org/10.1038/s41598-018-35916-0
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