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Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis
Heterosis can increase the yield of many crops and has been extensively applied in agriculture. In maize, female inflorescence architecture directly determines grain yield. Thus, exploring the relationship between early maize ear inflorescence development and heterosis regarding yield-related traits...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338801/ https://www.ncbi.nlm.nih.gov/pubmed/30659214 http://dx.doi.org/10.1038/s41598-018-36446-5 |
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author | Shi, Xia Zhang, Xuehai Shi, Dakun Zhang, Xiangge Li, Weihua Tang, Jihua |
author_facet | Shi, Xia Zhang, Xuehai Shi, Dakun Zhang, Xiangge Li, Weihua Tang, Jihua |
author_sort | Shi, Xia |
collection | PubMed |
description | Heterosis can increase the yield of many crops and has been extensively applied in agriculture. In maize, female inflorescence architecture directly determines grain yield. Thus, exploring the relationship between early maize ear inflorescence development and heterosis regarding yield-related traits may be helpful for characterizing the molecular mechanisms underlying heterotic performance. In this study, we fine mapped the overdominant heterotic locus (hlEW2b), associated with ear width, in an approximately 1.98-Mb region based on analyses of chromosome segment substitution lines and the corresponding testcross population. Maize ear inflorescences at the floral meristem stage were collected from two inbred lines, one chromosome segment substitution line that carried hlEW2b (sub-CSSL(16)), the receptor parent lx9801, and the Zheng58 × sub-CSSL(16) and Zheng58 × lx9801 hybrid lines. A total of 256 metabolites were identified, including 31 and 24 metabolites that were differentially accumulated between the two hybrid lines and between the two inbred lines, respectively. Most of these metabolites are involved in complex regulatory mechanisms important for maize ear development. For example, nucleotides are basic metabolites affecting cell composition and carbohydrate synthesis. Additionally, nicotinate and nicotinamide metabolism is important for photosynthesis, plant stress responses, and cell expansion. Moreover, flavonoid and phenolic metabolites regulate auxin transport and cell apoptosis. Meanwhile, phytohormone biosynthesis and distribution influence the cell cycle and cell proliferation. Our results revealed that changes in metabolite contents may affect the heterotic performance related to ear width and yield in maize hybrid lines. This study provides new clues in heterosis at the metabolomics level and implies that differentially accumulated metabolites made distinct contributions to the heterosis at an early stage of ear inflorescences development. |
format | Online Article Text |
id | pubmed-6338801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63388012019-01-23 Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis Shi, Xia Zhang, Xuehai Shi, Dakun Zhang, Xiangge Li, Weihua Tang, Jihua Sci Rep Article Heterosis can increase the yield of many crops and has been extensively applied in agriculture. In maize, female inflorescence architecture directly determines grain yield. Thus, exploring the relationship between early maize ear inflorescence development and heterosis regarding yield-related traits may be helpful for characterizing the molecular mechanisms underlying heterotic performance. In this study, we fine mapped the overdominant heterotic locus (hlEW2b), associated with ear width, in an approximately 1.98-Mb region based on analyses of chromosome segment substitution lines and the corresponding testcross population. Maize ear inflorescences at the floral meristem stage were collected from two inbred lines, one chromosome segment substitution line that carried hlEW2b (sub-CSSL(16)), the receptor parent lx9801, and the Zheng58 × sub-CSSL(16) and Zheng58 × lx9801 hybrid lines. A total of 256 metabolites were identified, including 31 and 24 metabolites that were differentially accumulated between the two hybrid lines and between the two inbred lines, respectively. Most of these metabolites are involved in complex regulatory mechanisms important for maize ear development. For example, nucleotides are basic metabolites affecting cell composition and carbohydrate synthesis. Additionally, nicotinate and nicotinamide metabolism is important for photosynthesis, plant stress responses, and cell expansion. Moreover, flavonoid and phenolic metabolites regulate auxin transport and cell apoptosis. Meanwhile, phytohormone biosynthesis and distribution influence the cell cycle and cell proliferation. Our results revealed that changes in metabolite contents may affect the heterotic performance related to ear width and yield in maize hybrid lines. This study provides new clues in heterosis at the metabolomics level and implies that differentially accumulated metabolites made distinct contributions to the heterosis at an early stage of ear inflorescences development. Nature Publishing Group UK 2019-01-18 /pmc/articles/PMC6338801/ /pubmed/30659214 http://dx.doi.org/10.1038/s41598-018-36446-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shi, Xia Zhang, Xuehai Shi, Dakun Zhang, Xiangge Li, Weihua Tang, Jihua Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title | Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title_full | Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title_fullStr | Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title_full_unstemmed | Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title_short | Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis |
title_sort | dissecting heterosis during the ear inflorescence development stage in maize via a metabolomics-based analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338801/ https://www.ncbi.nlm.nih.gov/pubmed/30659214 http://dx.doi.org/10.1038/s41598-018-36446-5 |
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