Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a n...

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Detalles Bibliográficos
Autores principales: Jeong, Hee-Jin, Dong, Jinhua, Ueda, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338965/
https://www.ncbi.nlm.nih.gov/pubmed/30583603
http://dx.doi.org/10.3390/s19010052
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author Jeong, Hee-Jin
Dong, Jinhua
Ueda, Hiroshi
author_facet Jeong, Hee-Jin
Dong, Jinhua
Ueda, Hiroshi
author_sort Jeong, Hee-Jin
collection PubMed
description We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.
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spelling pubmed-63389652019-01-23 Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies Jeong, Hee-Jin Dong, Jinhua Ueda, Hiroshi Sensors (Basel) Communication We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus. MDPI 2018-12-23 /pmc/articles/PMC6338965/ /pubmed/30583603 http://dx.doi.org/10.3390/s19010052 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Jeong, Hee-Jin
Dong, Jinhua
Ueda, Hiroshi
Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title_full Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title_fullStr Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title_full_unstemmed Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title_short Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
title_sort single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338965/
https://www.ncbi.nlm.nih.gov/pubmed/30583603
http://dx.doi.org/10.3390/s19010052
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AT uedahiroshi singlestepdetectionoftheinfluenzavirushemagglutininusingbacteriallyproducedquenchbodies

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