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Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3

Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatme...

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Autores principales: Cabello, Miguel, Ge, Haobo, Aracil, Carmen, Moschou, Despina, Estrela, Pedro, Manuel Quero, Jose, I. Pascu, Sofia, R. F. Rocha, Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339143/
https://www.ncbi.nlm.nih.gov/pubmed/30609788
http://dx.doi.org/10.3390/s19010139
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author Cabello, Miguel
Ge, Haobo
Aracil, Carmen
Moschou, Despina
Estrela, Pedro
Manuel Quero, Jose
I. Pascu, Sofia
R. F. Rocha, Paulo
author_facet Cabello, Miguel
Ge, Haobo
Aracil, Carmen
Moschou, Despina
Estrela, Pedro
Manuel Quero, Jose
I. Pascu, Sofia
R. F. Rocha, Paulo
author_sort Cabello, Miguel
collection PubMed
description Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatment strategies. Whilst electrophysiological studies can provide in depth analysis, the possibility of recording electrical activity in large populations of non-neuronal cells remains a significant challenge, even harder to address in the picoAmpere-range, which is typical of cellular level electrical activities. In this paper, we present the measurement and characterization of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA) with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with Gd(3)⁺, known as an inhibitor for the Ca(2)⁺ exchanges, suggest that the quasi-periodic signals originate from Ca(2)⁺ channels. After adding the Gd(3)⁺ to a population of living PC-3 cells, their electrical activity considerably decreased; once the culture was washed, thus eliminating the Gd(3)⁺ containing medium and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity. Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the use of Gd(3)⁺, on the timescale of this experiment. Hence, this experimental work suggests that Ca(2)⁺ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations. Our measuring platform opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways.
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spelling pubmed-63391432019-01-23 Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3 Cabello, Miguel Ge, Haobo Aracil, Carmen Moschou, Despina Estrela, Pedro Manuel Quero, Jose I. Pascu, Sofia R. F. Rocha, Paulo Sensors (Basel) Article Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatment strategies. Whilst electrophysiological studies can provide in depth analysis, the possibility of recording electrical activity in large populations of non-neuronal cells remains a significant challenge, even harder to address in the picoAmpere-range, which is typical of cellular level electrical activities. In this paper, we present the measurement and characterization of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA) with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with Gd(3)⁺, known as an inhibitor for the Ca(2)⁺ exchanges, suggest that the quasi-periodic signals originate from Ca(2)⁺ channels. After adding the Gd(3)⁺ to a population of living PC-3 cells, their electrical activity considerably decreased; once the culture was washed, thus eliminating the Gd(3)⁺ containing medium and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity. Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the use of Gd(3)⁺, on the timescale of this experiment. Hence, this experimental work suggests that Ca(2)⁺ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations. Our measuring platform opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways. MDPI 2019-01-03 /pmc/articles/PMC6339143/ /pubmed/30609788 http://dx.doi.org/10.3390/s19010139 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cabello, Miguel
Ge, Haobo
Aracil, Carmen
Moschou, Despina
Estrela, Pedro
Manuel Quero, Jose
I. Pascu, Sofia
R. F. Rocha, Paulo
Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title_full Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title_fullStr Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title_full_unstemmed Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title_short Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3
title_sort extracellular electrophysiology in the prostate cancer cell model pc-3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339143/
https://www.ncbi.nlm.nih.gov/pubmed/30609788
http://dx.doi.org/10.3390/s19010139
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