High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

BACKGROUND: Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. RESULTS: We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to...

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Autores principales: Gustafsson, Charlotte, De Paepe, Ayla, Schmidl, Christian, Månsson, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339279/
https://www.ncbi.nlm.nih.gov/pubmed/30658577
http://dx.doi.org/10.1186/s12864-018-5299-0
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author Gustafsson, Charlotte
De Paepe, Ayla
Schmidl, Christian
Månsson, Robert
author_facet Gustafsson, Charlotte
De Paepe, Ayla
Schmidl, Christian
Månsson, Robert
author_sort Gustafsson, Charlotte
collection PubMed
description BACKGROUND: Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. RESULTS: We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. CONCLUSIONS: The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5299-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-63392792019-01-23 High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers Gustafsson, Charlotte De Paepe, Ayla Schmidl, Christian Månsson, Robert BMC Genomics Methodology Article BACKGROUND: Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. RESULTS: We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. CONCLUSIONS: The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5299-0) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-18 /pmc/articles/PMC6339279/ /pubmed/30658577 http://dx.doi.org/10.1186/s12864-018-5299-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Gustafsson, Charlotte
De Paepe, Ayla
Schmidl, Christian
Månsson, Robert
High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title_full High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title_fullStr High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title_full_unstemmed High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title_short High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
title_sort high-throughput chipmentation: freely scalable, single day chipseq data generation from very low cell-numbers
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339279/
https://www.ncbi.nlm.nih.gov/pubmed/30658577
http://dx.doi.org/10.1186/s12864-018-5299-0
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