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A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin

For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a cardiac glycoside used for the treatment of tachycardia with a narrow therapeutic window of 0.5–2.0 ng...

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Autores principales: Ruppert, Christoph, Phogat, Navneet, Laufer, Stefan, Kohl, Matthias, Deigner, Hans-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339659/
https://www.ncbi.nlm.nih.gov/pubmed/30661134
http://dx.doi.org/10.1007/s00604-018-3195-6
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author Ruppert, Christoph
Phogat, Navneet
Laufer, Stefan
Kohl, Matthias
Deigner, Hans-Peter
author_facet Ruppert, Christoph
Phogat, Navneet
Laufer, Stefan
Kohl, Matthias
Deigner, Hans-Peter
author_sort Ruppert, Christoph
collection PubMed
description For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a cardiac glycoside used for the treatment of tachycardia with a narrow therapeutic window of 0.5–2.0 ng·mL(−1), and toxic effects are common for concentrations above 2.5 ng·mL(−1). For monitoring of blood concentration levels and treatment of intoxication, highly selective antibodies for digoxin and its hapten, digoxigenin, are available. A smartphone readout system is described for measuring digoxigenin in human serum using a common gold nanoparticle lateral flow assay (LFA). The R-package GNSplex, which also includes a Shiny app for quantitative test interpretation based on linear models, is used for image analysis. Images of lateral flow strips were taken with an iPhone camera and a simple darkbox made from black cardboard. Sensitivity and accuracy of the quantitative smartphone system as well as analytical parameters such as limit of detection (LOD) were determined and compared to data obtained with a high resolution BioImager. The data show that the smartphone based digoxin assay yields reliable quantitative results within the clinically relevant concentration range. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00604-018-3195-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-63396592019-02-01 A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin Ruppert, Christoph Phogat, Navneet Laufer, Stefan Kohl, Matthias Deigner, Hans-Peter Mikrochim Acta Original Paper For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a cardiac glycoside used for the treatment of tachycardia with a narrow therapeutic window of 0.5–2.0 ng·mL(−1), and toxic effects are common for concentrations above 2.5 ng·mL(−1). For monitoring of blood concentration levels and treatment of intoxication, highly selective antibodies for digoxin and its hapten, digoxigenin, are available. A smartphone readout system is described for measuring digoxigenin in human serum using a common gold nanoparticle lateral flow assay (LFA). The R-package GNSplex, which also includes a Shiny app for quantitative test interpretation based on linear models, is used for image analysis. Images of lateral flow strips were taken with an iPhone camera and a simple darkbox made from black cardboard. Sensitivity and accuracy of the quantitative smartphone system as well as analytical parameters such as limit of detection (LOD) were determined and compared to data obtained with a high resolution BioImager. The data show that the smartphone based digoxin assay yields reliable quantitative results within the clinically relevant concentration range. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00604-018-3195-6) contains supplementary material, which is available to authorized users. Springer Vienna 2019-01-19 2019 /pmc/articles/PMC6339659/ /pubmed/30661134 http://dx.doi.org/10.1007/s00604-018-3195-6 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Ruppert, Christoph
Phogat, Navneet
Laufer, Stefan
Kohl, Matthias
Deigner, Hans-Peter
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title_full A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title_fullStr A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title_full_unstemmed A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title_short A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
title_sort smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339659/
https://www.ncbi.nlm.nih.gov/pubmed/30661134
http://dx.doi.org/10.1007/s00604-018-3195-6
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