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Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran

BACKGROUND AND OBJECTIVES: Antibiotic resistance in Pseudomonas aeruginosa is an increasing health problem. Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibiotics. This study aimed at screening the presence of class 1, 2 and 3 integrons...

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Autores principales: Zarei-Yazdeli, Mohadeseh, Eslami, Gilda, Zandi, Hengameh, Kiani, Masoumeh, Barzegar, Kazem, Alipanah, Hanieh, Mousavi, Seyed Morteza, Shukohifar, Marzieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340001/
https://www.ncbi.nlm.nih.gov/pubmed/30675326
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author Zarei-Yazdeli, Mohadeseh
Eslami, Gilda
Zandi, Hengameh
Kiani, Masoumeh
Barzegar, Kazem
Alipanah, Hanieh
Mousavi, Seyed Morteza
Shukohifar, Marzieh
author_facet Zarei-Yazdeli, Mohadeseh
Eslami, Gilda
Zandi, Hengameh
Kiani, Masoumeh
Barzegar, Kazem
Alipanah, Hanieh
Mousavi, Seyed Morteza
Shukohifar, Marzieh
author_sort Zarei-Yazdeli, Mohadeseh
collection PubMed
description BACKGROUND AND OBJECTIVES: Antibiotic resistance in Pseudomonas aeruginosa is an increasing health problem. Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibiotics. This study aimed at screening the presence of class 1, 2 and 3 integrons in P. aeruginosa in Yazd, Iran. MATERIALS AND METHODS: This study was carried out on P. aeruginosa strains from March 2016 to March 2017. Clinical specimens were initially identified by the standard biochemical methods and their resistance patterns to antibiotics were studied using the disc diffusion method. PCR was carried out for the detection of class 1, 2 and 3 integrons using intI1, intI2 and intI3 gene primers, respectively. RESULTS: Antimicrobial susceptibility test showed that 75% of isolates were detected as multi-drug resistant (MDR), and lowest resistance was observed in ciprofloxacin (48.6%) and most resistance was in gentamicin (63.2%). Moreover, PCR results showed that 22 (15.3%) and 119 (82.6%) of P. aeruginosa isolates carried intI2 and intI1 genes, but intI3 gene was not found. CONCLUSION: Since it is customary to observe Class I integrons in P. aeruginosa isolated from clinical samples, they are often responsible for antibiotic resistance gene transfer, which calls for evaluation of integrons as contributing factors in antibiotic resistance.
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spelling pubmed-63400012019-01-23 Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran Zarei-Yazdeli, Mohadeseh Eslami, Gilda Zandi, Hengameh Kiani, Masoumeh Barzegar, Kazem Alipanah, Hanieh Mousavi, Seyed Morteza Shukohifar, Marzieh Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Antibiotic resistance in Pseudomonas aeruginosa is an increasing health problem. Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibiotics. This study aimed at screening the presence of class 1, 2 and 3 integrons in P. aeruginosa in Yazd, Iran. MATERIALS AND METHODS: This study was carried out on P. aeruginosa strains from March 2016 to March 2017. Clinical specimens were initially identified by the standard biochemical methods and their resistance patterns to antibiotics were studied using the disc diffusion method. PCR was carried out for the detection of class 1, 2 and 3 integrons using intI1, intI2 and intI3 gene primers, respectively. RESULTS: Antimicrobial susceptibility test showed that 75% of isolates were detected as multi-drug resistant (MDR), and lowest resistance was observed in ciprofloxacin (48.6%) and most resistance was in gentamicin (63.2%). Moreover, PCR results showed that 22 (15.3%) and 119 (82.6%) of P. aeruginosa isolates carried intI2 and intI1 genes, but intI3 gene was not found. CONCLUSION: Since it is customary to observe Class I integrons in P. aeruginosa isolated from clinical samples, they are often responsible for antibiotic resistance gene transfer, which calls for evaluation of integrons as contributing factors in antibiotic resistance. Tehran University of Medical Sciences 2018-10 /pmc/articles/PMC6340001/ /pubmed/30675326 Text en Copyright© 2018 Iranian Neuroscience Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zarei-Yazdeli, Mohadeseh
Eslami, Gilda
Zandi, Hengameh
Kiani, Masoumeh
Barzegar, Kazem
Alipanah, Hanieh
Mousavi, Seyed Morteza
Shukohifar, Marzieh
Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title_full Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title_fullStr Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title_full_unstemmed Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title_short Prevalence of class 1, 2 and 3 integrons among multidrug-resistant Pseudomonas aeruginosa in Yazd, Iran
title_sort prevalence of class 1, 2 and 3 integrons among multidrug-resistant pseudomonas aeruginosa in yazd, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340001/
https://www.ncbi.nlm.nih.gov/pubmed/30675326
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