Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines

Wolbachia suppresses the replication of +ssRNA viruses such as dengue and Zika viruses in Aedes aegypti mosquitoes. However, the range of viruses affected by this endosymbiont is yet to be explored. Recently, novel insect-specific viruses (ISVs) have been described from numerous mosquito species and...

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Autores principales: McLean, Breeanna J., Dainty, Kimberley R., Flores, Heather A., O’Neill, Scott L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340807/
https://www.ncbi.nlm.nih.gov/pubmed/30503908
http://dx.doi.org/10.1016/j.virol.2018.11.012
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author McLean, Breeanna J.
Dainty, Kimberley R.
Flores, Heather A.
O’Neill, Scott L.
author_facet McLean, Breeanna J.
Dainty, Kimberley R.
Flores, Heather A.
O’Neill, Scott L.
author_sort McLean, Breeanna J.
collection PubMed
description Wolbachia suppresses the replication of +ssRNA viruses such as dengue and Zika viruses in Aedes aegypti mosquitoes. However, the range of viruses affected by this endosymbiont is yet to be explored. Recently, novel insect-specific viruses (ISVs) have been described from numerous mosquito species and mosquito-derived cell lines. Cell-fusing agent virus (Flaviviridae) and Phasi Charoen-like virus (Bunyaviridae) persistently infect the Ae. aegypti cell line Aag2 which has been used for experimental studies with both the wMel and wMelPop-CLA strains. Wolbachia was found to restrict the replication of CFAV but not the PCLV infection in these lines. Furthermore, an additional Ae. albopictus cell line (RML-12) which contained either wMel or wMelPop-CLA was assessed. While no infectious +ssRNA or dsRNA viruses were detected, a PCLV infection was identified. These observations provide additional evidence to support that insect-specific, +ssRNA viruses can be suppressed in cell culture by Wolbachia but -ssRNA viruses may not.
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spelling pubmed-63408072019-01-24 Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines McLean, Breeanna J. Dainty, Kimberley R. Flores, Heather A. O’Neill, Scott L. Virology Article Wolbachia suppresses the replication of +ssRNA viruses such as dengue and Zika viruses in Aedes aegypti mosquitoes. However, the range of viruses affected by this endosymbiont is yet to be explored. Recently, novel insect-specific viruses (ISVs) have been described from numerous mosquito species and mosquito-derived cell lines. Cell-fusing agent virus (Flaviviridae) and Phasi Charoen-like virus (Bunyaviridae) persistently infect the Ae. aegypti cell line Aag2 which has been used for experimental studies with both the wMel and wMelPop-CLA strains. Wolbachia was found to restrict the replication of CFAV but not the PCLV infection in these lines. Furthermore, an additional Ae. albopictus cell line (RML-12) which contained either wMel or wMelPop-CLA was assessed. While no infectious +ssRNA or dsRNA viruses were detected, a PCLV infection was identified. These observations provide additional evidence to support that insect-specific, +ssRNA viruses can be suppressed in cell culture by Wolbachia but -ssRNA viruses may not. Academic Press 2019-01-15 /pmc/articles/PMC6340807/ /pubmed/30503908 http://dx.doi.org/10.1016/j.virol.2018.11.012 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
McLean, Breeanna J.
Dainty, Kimberley R.
Flores, Heather A.
O’Neill, Scott L.
Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title_full Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title_fullStr Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title_full_unstemmed Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title_short Differential suppression of persistent insect specific viruses in trans-infected wMel and wMelPop-CLA Aedes-derived mosquito lines
title_sort differential suppression of persistent insect specific viruses in trans-infected wmel and wmelpop-cla aedes-derived mosquito lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340807/
https://www.ncbi.nlm.nih.gov/pubmed/30503908
http://dx.doi.org/10.1016/j.virol.2018.11.012
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