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Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate

Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-ele...

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Autores principales: Zhou, Dejian, Zhu, Xing, Zheng, Sanduo, Tan, Dan, Dong, Meng-Qiu, Ye, Keqiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Higher Education Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340896/
https://www.ncbi.nlm.nih.gov/pubmed/29557065
http://dx.doi.org/10.1007/s13238-018-0526-7
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author Zhou, Dejian
Zhu, Xing
Zheng, Sanduo
Tan, Dan
Dong, Meng-Qiu
Ye, Keqiong
author_facet Zhou, Dejian
Zhu, Xing
Zheng, Sanduo
Tan, Dan
Dong, Meng-Qiu
Ye, Keqiong
author_sort Zhou, Dejian
collection PubMed
description Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13238-018-0526-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-63408962019-02-06 Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate Zhou, Dejian Zhu, Xing Zheng, Sanduo Tan, Dan Dong, Meng-Qiu Ye, Keqiong Protein Cell Research Article Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13238-018-0526-7) contains supplementary material, which is available to authorized users. Higher Education Press 2018-03-19 2019-02 /pmc/articles/PMC6340896/ /pubmed/29557065 http://dx.doi.org/10.1007/s13238-018-0526-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Article
Zhou, Dejian
Zhu, Xing
Zheng, Sanduo
Tan, Dan
Dong, Meng-Qiu
Ye, Keqiong
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title_full Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title_fullStr Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title_full_unstemmed Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title_short Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
title_sort cryo-em structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340896/
https://www.ncbi.nlm.nih.gov/pubmed/29557065
http://dx.doi.org/10.1007/s13238-018-0526-7
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