Cargando…
The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic eff...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340952/ https://www.ncbi.nlm.nih.gov/pubmed/30573530 http://dx.doi.org/10.1042/BSR20182201 |
_version_ | 1783388862702157824 |
---|---|
author | Li, Shaoli Xue, Guanhua Zhao, Hanqing Feng, Yanling Yan, Chao Cui, Jinghua Sun, Hongmei |
author_facet | Li, Shaoli Xue, Guanhua Zhao, Hanqing Feng, Yanling Yan, Chao Cui, Jinghua Sun, Hongmei |
author_sort | Li, Shaoli |
collection | PubMed |
description | Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic effects of HapE have not been explored. The present study examined the effects of this enzyme on normal human bronchial epithelial (NHBE) cells, in an attempt to identify additional mechanisms of M. pneumoniae pathogenesis. Recombinant HapE was purified for use in downstream assays. MTT and colony formation assays were conducted to determine the effects of HapE on cell viability and growth, while flow cytometry was used to examine changes in cell proliferation and cell cycle function. ELISA was performed to examine changes in the cytokine profile of HapE-treated cells. HapE treatment arrested NHBE cells in S phase and inhibited cell proliferation in a concentration-dependent manner. The anti-inflammatory factors interleukin (IL)-4 and IL-6 were significantly enhanced following HapE treatment. Increased secretion of pro-inflammatory factors was not observed. The effects of HapE on the respiratory epithelium may have an impact on the efficiency of host immune surveillance and pathogen elimination, and contribute to the pathogenesis of M. pneumoniae. |
format | Online Article Text |
id | pubmed-6340952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63409522019-01-28 The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells Li, Shaoli Xue, Guanhua Zhao, Hanqing Feng, Yanling Yan, Chao Cui, Jinghua Sun, Hongmei Biosci Rep Research Articles Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic effects of HapE have not been explored. The present study examined the effects of this enzyme on normal human bronchial epithelial (NHBE) cells, in an attempt to identify additional mechanisms of M. pneumoniae pathogenesis. Recombinant HapE was purified for use in downstream assays. MTT and colony formation assays were conducted to determine the effects of HapE on cell viability and growth, while flow cytometry was used to examine changes in cell proliferation and cell cycle function. ELISA was performed to examine changes in the cytokine profile of HapE-treated cells. HapE treatment arrested NHBE cells in S phase and inhibited cell proliferation in a concentration-dependent manner. The anti-inflammatory factors interleukin (IL)-4 and IL-6 were significantly enhanced following HapE treatment. Increased secretion of pro-inflammatory factors was not observed. The effects of HapE on the respiratory epithelium may have an impact on the efficiency of host immune surveillance and pathogen elimination, and contribute to the pathogenesis of M. pneumoniae. Portland Press Ltd. 2019-01-18 /pmc/articles/PMC6340952/ /pubmed/30573530 http://dx.doi.org/10.1042/BSR20182201 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Li, Shaoli Xue, Guanhua Zhao, Hanqing Feng, Yanling Yan, Chao Cui, Jinghua Sun, Hongmei The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title | The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title_full | The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title_fullStr | The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title_full_unstemmed | The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title_short | The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells |
title_sort | mycoplasma pneumoniae hape alters the cytokine profile and growth of human bronchial epithelial cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340952/ https://www.ncbi.nlm.nih.gov/pubmed/30573530 http://dx.doi.org/10.1042/BSR20182201 |
work_keys_str_mv | AT lishaoli themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT xueguanhua themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT zhaohanqing themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT fengyanling themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT yanchao themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT cuijinghua themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT sunhongmei themycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT lishaoli mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT xueguanhua mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT zhaohanqing mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT fengyanling mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT yanchao mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT cuijinghua mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells AT sunhongmei mycoplasmapneumoniaehapealtersthecytokineprofileandgrowthofhumanbronchialepithelialcells |