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The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells

Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic eff...

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Autores principales: Li, Shaoli, Xue, Guanhua, Zhao, Hanqing, Feng, Yanling, Yan, Chao, Cui, Jinghua, Sun, Hongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340952/
https://www.ncbi.nlm.nih.gov/pubmed/30573530
http://dx.doi.org/10.1042/BSR20182201
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author Li, Shaoli
Xue, Guanhua
Zhao, Hanqing
Feng, Yanling
Yan, Chao
Cui, Jinghua
Sun, Hongmei
author_facet Li, Shaoli
Xue, Guanhua
Zhao, Hanqing
Feng, Yanling
Yan, Chao
Cui, Jinghua
Sun, Hongmei
author_sort Li, Shaoli
collection PubMed
description Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic effects of HapE have not been explored. The present study examined the effects of this enzyme on normal human bronchial epithelial (NHBE) cells, in an attempt to identify additional mechanisms of M. pneumoniae pathogenesis. Recombinant HapE was purified for use in downstream assays. MTT and colony formation assays were conducted to determine the effects of HapE on cell viability and growth, while flow cytometry was used to examine changes in cell proliferation and cell cycle function. ELISA was performed to examine changes in the cytokine profile of HapE-treated cells. HapE treatment arrested NHBE cells in S phase and inhibited cell proliferation in a concentration-dependent manner. The anti-inflammatory factors interleukin (IL)-4 and IL-6 were significantly enhanced following HapE treatment. Increased secretion of pro-inflammatory factors was not observed. The effects of HapE on the respiratory epithelium may have an impact on the efficiency of host immune surveillance and pathogen elimination, and contribute to the pathogenesis of M. pneumoniae.
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spelling pubmed-63409522019-01-28 The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells Li, Shaoli Xue, Guanhua Zhao, Hanqing Feng, Yanling Yan, Chao Cui, Jinghua Sun, Hongmei Biosci Rep Research Articles Mycoplasma pneumoniae is one of the most common pathogenic causes of community-acquired pneumonia. Hydrogen sulfide, alanine, and pyruvate producing enzyme (HapE) is a recently discovered M. pneumoniae virulence factor that can produce H(2)S to promote erythrocyte lysis. However, other cytotoxic effects of HapE have not been explored. The present study examined the effects of this enzyme on normal human bronchial epithelial (NHBE) cells, in an attempt to identify additional mechanisms of M. pneumoniae pathogenesis. Recombinant HapE was purified for use in downstream assays. MTT and colony formation assays were conducted to determine the effects of HapE on cell viability and growth, while flow cytometry was used to examine changes in cell proliferation and cell cycle function. ELISA was performed to examine changes in the cytokine profile of HapE-treated cells. HapE treatment arrested NHBE cells in S phase and inhibited cell proliferation in a concentration-dependent manner. The anti-inflammatory factors interleukin (IL)-4 and IL-6 were significantly enhanced following HapE treatment. Increased secretion of pro-inflammatory factors was not observed. The effects of HapE on the respiratory epithelium may have an impact on the efficiency of host immune surveillance and pathogen elimination, and contribute to the pathogenesis of M. pneumoniae. Portland Press Ltd. 2019-01-18 /pmc/articles/PMC6340952/ /pubmed/30573530 http://dx.doi.org/10.1042/BSR20182201 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Li, Shaoli
Xue, Guanhua
Zhao, Hanqing
Feng, Yanling
Yan, Chao
Cui, Jinghua
Sun, Hongmei
The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title_full The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title_fullStr The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title_full_unstemmed The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title_short The Mycoplasma pneumoniae HapE alters the cytokine profile and growth of human bronchial epithelial cells
title_sort mycoplasma pneumoniae hape alters the cytokine profile and growth of human bronchial epithelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340952/
https://www.ncbi.nlm.nih.gov/pubmed/30573530
http://dx.doi.org/10.1042/BSR20182201
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