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CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases
Plant pathogens like virus, bacteria, and fungi incur a huge loss of global productivity. Targeting the dominant R gene resulted in the evolution of resistance in pathogens, which shifted plant pathologists’ attention toward host susceptibility factors (or S genes). Herein, the application of sequen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341021/ https://www.ncbi.nlm.nih.gov/pubmed/30697226 http://dx.doi.org/10.3389/fpls.2018.02008 |
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author | Das, Ayan Sharma, Namisha Prasad, Manoj |
author_facet | Das, Ayan Sharma, Namisha Prasad, Manoj |
author_sort | Das, Ayan |
collection | PubMed |
description | Plant pathogens like virus, bacteria, and fungi incur a huge loss of global productivity. Targeting the dominant R gene resulted in the evolution of resistance in pathogens, which shifted plant pathologists’ attention toward host susceptibility factors (or S genes). Herein, the application of sequence-specific nucleases (SSNs) for targeted genome editing are gaining more importance, which utilize the use of meganucleases (MN), zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN) with the latest one namely clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The first generation of genome editing technologies, due to their cumbersome nature, is becoming obsolete. Owing to its simple and inexpensive nature the use of CRISPR/Cas9 system has revolutionized targeted genome editing technology. CRISPR/Cas9 system has been exploited for developing resistance against virus, bacteria, and fungi. For resistance to DNA viruses (mainly single-stranded DNA viruses), different parts of the viral genome have been targeted transiently and by the development of transgenic plants. For RNA viruses, mainly the host susceptibility factors and very recently the viral RNA genome itself have been targeted. Fungal and bacterial resistance has been achieved mainly by targeting the host susceptibility genes through the development of transgenics. In spite of these successes CRISPR/Cas9 system suffers from off-targeting. This and other problems associated with this system are being tackled by the continuous discovery/evolution of new variants. Finally, the regulatory standpoint regarding CRISPR/Cas9 will determine the fate of using this versatile tool in developing pathogen resistance in crop plants. |
format | Online Article Text |
id | pubmed-6341021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63410212019-01-29 CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases Das, Ayan Sharma, Namisha Prasad, Manoj Front Plant Sci Plant Science Plant pathogens like virus, bacteria, and fungi incur a huge loss of global productivity. Targeting the dominant R gene resulted in the evolution of resistance in pathogens, which shifted plant pathologists’ attention toward host susceptibility factors (or S genes). Herein, the application of sequence-specific nucleases (SSNs) for targeted genome editing are gaining more importance, which utilize the use of meganucleases (MN), zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN) with the latest one namely clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The first generation of genome editing technologies, due to their cumbersome nature, is becoming obsolete. Owing to its simple and inexpensive nature the use of CRISPR/Cas9 system has revolutionized targeted genome editing technology. CRISPR/Cas9 system has been exploited for developing resistance against virus, bacteria, and fungi. For resistance to DNA viruses (mainly single-stranded DNA viruses), different parts of the viral genome have been targeted transiently and by the development of transgenic plants. For RNA viruses, mainly the host susceptibility factors and very recently the viral RNA genome itself have been targeted. Fungal and bacterial resistance has been achieved mainly by targeting the host susceptibility genes through the development of transgenics. In spite of these successes CRISPR/Cas9 system suffers from off-targeting. This and other problems associated with this system are being tackled by the continuous discovery/evolution of new variants. Finally, the regulatory standpoint regarding CRISPR/Cas9 will determine the fate of using this versatile tool in developing pathogen resistance in crop plants. Frontiers Media S.A. 2019-01-15 /pmc/articles/PMC6341021/ /pubmed/30697226 http://dx.doi.org/10.3389/fpls.2018.02008 Text en Copyright © 2019 Das, Sharma and Prasad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Das, Ayan Sharma, Namisha Prasad, Manoj CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title | CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title_full | CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title_fullStr | CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title_full_unstemmed | CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title_short | CRISPR/Cas9: A Novel Weapon in the Arsenal to Combat Plant Diseases |
title_sort | crispr/cas9: a novel weapon in the arsenal to combat plant diseases |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341021/ https://www.ncbi.nlm.nih.gov/pubmed/30697226 http://dx.doi.org/10.3389/fpls.2018.02008 |
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