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Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut

Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐g...

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Detalles Bibliográficos
Autores principales: Afouda, Pamela, Durand, Guillaume A., Lagier, Jean‐Christophe, Labas, Noémie, Cadoret, Fréderic, Armstrong, Nicholas, Raoult, Didier, Dubourg, Grégory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341035/
https://www.ncbi.nlm.nih.gov/pubmed/29654664
http://dx.doi.org/10.1002/mbo3.621
Descripción
Sumario:Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐genomics, it is strictly anaerobic, nonspore‐forming, rod‐shaped, with catalase‐ and oxidase‐negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein‐coding genes and 62 RNA‐coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.