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Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐g...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341035/ https://www.ncbi.nlm.nih.gov/pubmed/29654664 http://dx.doi.org/10.1002/mbo3.621 |
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author | Afouda, Pamela Durand, Guillaume A. Lagier, Jean‐Christophe Labas, Noémie Cadoret, Fréderic Armstrong, Nicholas Raoult, Didier Dubourg, Grégory |
author_facet | Afouda, Pamela Durand, Guillaume A. Lagier, Jean‐Christophe Labas, Noémie Cadoret, Fréderic Armstrong, Nicholas Raoult, Didier Dubourg, Grégory |
author_sort | Afouda, Pamela |
collection | PubMed |
description | Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐genomics, it is strictly anaerobic, nonspore‐forming, rod‐shaped, with catalase‐ and oxidase‐negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein‐coding genes and 62 RNA‐coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal. |
format | Online Article Text |
id | pubmed-6341035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63410352019-01-24 Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut Afouda, Pamela Durand, Guillaume A. Lagier, Jean‐Christophe Labas, Noémie Cadoret, Fréderic Armstrong, Nicholas Raoult, Didier Dubourg, Grégory Microbiologyopen Original Research Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐genomics, it is strictly anaerobic, nonspore‐forming, rod‐shaped, with catalase‐ and oxidase‐negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein‐coding genes and 62 RNA‐coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal. John Wiley and Sons Inc. 2018-04-14 /pmc/articles/PMC6341035/ /pubmed/29654664 http://dx.doi.org/10.1002/mbo3.621 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Afouda, Pamela Durand, Guillaume A. Lagier, Jean‐Christophe Labas, Noémie Cadoret, Fréderic Armstrong, Nicholas Raoult, Didier Dubourg, Grégory Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title | Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title_full | Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title_fullStr | Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title_full_unstemmed | Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title_short | Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut |
title_sort | noncontiguous finished genome sequence and description of intestinimonas massiliensis sp. nov strain gd2(t), the second intestinimonas species cultured from the human gut |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341035/ https://www.ncbi.nlm.nih.gov/pubmed/29654664 http://dx.doi.org/10.1002/mbo3.621 |
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