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Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut

Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐g...

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Autores principales: Afouda, Pamela, Durand, Guillaume A., Lagier, Jean‐Christophe, Labas, Noémie, Cadoret, Fréderic, Armstrong, Nicholas, Raoult, Didier, Dubourg, Grégory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341035/
https://www.ncbi.nlm.nih.gov/pubmed/29654664
http://dx.doi.org/10.1002/mbo3.621
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author Afouda, Pamela
Durand, Guillaume A.
Lagier, Jean‐Christophe
Labas, Noémie
Cadoret, Fréderic
Armstrong, Nicholas
Raoult, Didier
Dubourg, Grégory
author_facet Afouda, Pamela
Durand, Guillaume A.
Lagier, Jean‐Christophe
Labas, Noémie
Cadoret, Fréderic
Armstrong, Nicholas
Raoult, Didier
Dubourg, Grégory
author_sort Afouda, Pamela
collection PubMed
description Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐genomics, it is strictly anaerobic, nonspore‐forming, rod‐shaped, with catalase‐ and oxidase‐negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein‐coding genes and 62 RNA‐coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal.
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spelling pubmed-63410352019-01-24 Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut Afouda, Pamela Durand, Guillaume A. Lagier, Jean‐Christophe Labas, Noémie Cadoret, Fréderic Armstrong, Nicholas Raoult, Didier Dubourg, Grégory Microbiologyopen Original Research Intestinimonas massiliensis sp. nov strain GD2(T) is a new species of the genus Intestinimonas (the second, following Intestinimonas butyriciproducens gen. nov., sp. nov). First isolated from the gut microbiota of a healthy subject of French origin using a culturomics approach combined with taxono‐genomics, it is strictly anaerobic, nonspore‐forming, rod‐shaped, with catalase‐ and oxidase‐negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched with sheep blood (5%) and rumen fluid (5%), incubated at 37°C. Its phenotypic and genotypic descriptions are presented in this paper with a full annotation of its genome sequence. This genome consists of 3,104,261 bp in length and contains 3,074 predicted genes, including 3,012 protein‐coding genes and 62 RNA‐coding genes. Strain GD2(T) significantly produces butyrate and is frequently found among available 16S rRNA gene amplicon datasets, which leads consideration of Intestinimonas massiliensis as an important human gut commensal. John Wiley and Sons Inc. 2018-04-14 /pmc/articles/PMC6341035/ /pubmed/29654664 http://dx.doi.org/10.1002/mbo3.621 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Afouda, Pamela
Durand, Guillaume A.
Lagier, Jean‐Christophe
Labas, Noémie
Cadoret, Fréderic
Armstrong, Nicholas
Raoult, Didier
Dubourg, Grégory
Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title_full Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title_fullStr Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title_full_unstemmed Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title_short Noncontiguous finished genome sequence and description of Intestinimonas massiliensis sp. nov strain GD2(T), the second Intestinimonas species cultured from the human gut
title_sort noncontiguous finished genome sequence and description of intestinimonas massiliensis sp. nov strain gd2(t), the second intestinimonas species cultured from the human gut
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341035/
https://www.ncbi.nlm.nih.gov/pubmed/29654664
http://dx.doi.org/10.1002/mbo3.621
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