Cargando…

Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells

The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the react...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Wen-Xue, Li, Jin-Gao, Wan, Xiang-Hui, Zou, Xue-Sen, Qi, Shu-Yi, Zhang, Yu-Qing, Weng, Qiu-Min, Li, Jun-Yu, Xiong, Wen-Min, Xie, Chen, Cheng, Wei-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341559/
https://www.ncbi.nlm.nih.gov/pubmed/30675216
http://dx.doi.org/10.3892/ol.2018.9771
_version_ 1783388964410884096
author Chen, Wen-Xue
Li, Jin-Gao
Wan, Xiang-Hui
Zou, Xue-Sen
Qi, Shu-Yi
Zhang, Yu-Qing
Weng, Qiu-Min
Li, Jun-Yu
Xiong, Wen-Min
Xie, Chen
Cheng, Wei-Liang
author_facet Chen, Wen-Xue
Li, Jin-Gao
Wan, Xiang-Hui
Zou, Xue-Sen
Qi, Shu-Yi
Zhang, Yu-Qing
Weng, Qiu-Min
Li, Jun-Yu
Xiong, Wen-Min
Xie, Chen
Cheng, Wei-Liang
author_sort Chen, Wen-Xue
collection PubMed
description The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the reaction chamber (consisting of columns) using a biotin-avidin system. Cell suspension was introduced into the reaction chamber to capture NPC cells. NPC cells were subsequently eluted, and the capture rate of the cells was calculated. The modified aptamer-bound microfluidic chip was able to capture NPC cells with a capture rate of ~90%. The modified aptamer-bound microfluidic chip has a wide range of potential applications for the diagnosis of NPC.
format Online
Article
Text
id pubmed-6341559
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-63415592019-01-23 Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells Chen, Wen-Xue Li, Jin-Gao Wan, Xiang-Hui Zou, Xue-Sen Qi, Shu-Yi Zhang, Yu-Qing Weng, Qiu-Min Li, Jun-Yu Xiong, Wen-Min Xie, Chen Cheng, Wei-Liang Oncol Lett Articles The aim of the present study was to discuss the design of a microfluidic chip consisting of columns, and its use for the enrichment of nasopharyngeal cancer (NPC) cells. A microfluidic chip experiment was simulated using FLUENT software. Within the microfluidic chip, aptamers were bound to the reaction chamber (consisting of columns) using a biotin-avidin system. Cell suspension was introduced into the reaction chamber to capture NPC cells. NPC cells were subsequently eluted, and the capture rate of the cells was calculated. The modified aptamer-bound microfluidic chip was able to capture NPC cells with a capture rate of ~90%. The modified aptamer-bound microfluidic chip has a wide range of potential applications for the diagnosis of NPC. D.A. Spandidos 2019-02 2018-11-28 /pmc/articles/PMC6341559/ /pubmed/30675216 http://dx.doi.org/10.3892/ol.2018.9771 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Wen-Xue
Li, Jin-Gao
Wan, Xiang-Hui
Zou, Xue-Sen
Qi, Shu-Yi
Zhang, Yu-Qing
Weng, Qiu-Min
Li, Jun-Yu
Xiong, Wen-Min
Xie, Chen
Cheng, Wei-Liang
Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title_full Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title_fullStr Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title_full_unstemmed Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title_short Design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
title_sort design of a microfluidic chip consisting of micropillars and its use for the enrichment of nasopharyngeal cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341559/
https://www.ncbi.nlm.nih.gov/pubmed/30675216
http://dx.doi.org/10.3892/ol.2018.9771
work_keys_str_mv AT chenwenxue designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT lijingao designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT wanxianghui designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT zouxuesen designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT qishuyi designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT zhangyuqing designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT wengqiumin designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT lijunyu designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT xiongwenmin designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT xiechen designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells
AT chengweiliang designofamicrofluidicchipconsistingofmicropillarsanditsusefortheenrichmentofnasopharyngealcancercells