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Expression profile and biological function of miR-455-5p in colorectal carcinoma

Underexpression of microRNA-455-5p (miR-455-5p) in medullary thyroid carcinoma, melanoma, gastric cancer and additional cancer types has been reported, which may be associated with carcinoma development. The present study aimed to evaluate the expression profile and biological role of miR-455-5p in...

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Autores principales: Wang, Jinqiu, Lu, Yang, Zeng, Yiyong, Zhang, Leming, Ke, Kongliang, Guo, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341642/
https://www.ncbi.nlm.nih.gov/pubmed/30675279
http://dx.doi.org/10.3892/ol.2018.9862
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author Wang, Jinqiu
Lu, Yang
Zeng, Yiyong
Zhang, Leming
Ke, Kongliang
Guo, Yu
author_facet Wang, Jinqiu
Lu, Yang
Zeng, Yiyong
Zhang, Leming
Ke, Kongliang
Guo, Yu
author_sort Wang, Jinqiu
collection PubMed
description Underexpression of microRNA-455-5p (miR-455-5p) in medullary thyroid carcinoma, melanoma, gastric cancer and additional cancer types has been reported, which may be associated with carcinoma development. The present study aimed to evaluate the expression profile and biological role of miR-455-5p in colorectal carcinoma. Carcinoma tissues and adjacent tissue specimens from 40 patients with colorectal cancer were randomly collected. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was conducted to detect the expression levels of miR-455-5p in colorectal carcinoma and adjacent normal tissues. The biological effects of miR-455-5p on selected colorectal cancer cells were assessed using bromodeoxyuridine assays, wound healing migration assays and flow cytometry. Bioinformatics analysis was implemented to predict the potential target genes of miR-455-5p in colorectal cancer. The expression levels of target genes were further validated by RT-qPCR and western blot analysis of the mRNA and protein levels. The results of the experiments demonstrated that miR-455-5p expression was downregulated in colorectal cancer tissues compared with adjacent normal tissues. In colorectal cancer cells (SW-480, HT-29 and HCT-116), miR-455-5p was observed to inhibit cell proliferation and migration while promoting cell apoptosis. Bioinformatics analysis predicted that the oncogene phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) was one of the top ranked target genes of miR-455-5p in colorectal cancer cells. This association was validated by RT-qPCR and western blotting. In vivo studies revealed that the expression level of miR-455-5p was significantly downregulated in human colorectal cancer. Further in vitro studies suggested that miR-455-5p may prevent the development of colorectal cancer by downregulating the oncogene PIK3R1. It was concluded that miR-455-5p may target and downregulate PIK3R1 in colorectal cancer.
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spelling pubmed-63416422019-01-23 Expression profile and biological function of miR-455-5p in colorectal carcinoma Wang, Jinqiu Lu, Yang Zeng, Yiyong Zhang, Leming Ke, Kongliang Guo, Yu Oncol Lett Articles Underexpression of microRNA-455-5p (miR-455-5p) in medullary thyroid carcinoma, melanoma, gastric cancer and additional cancer types has been reported, which may be associated with carcinoma development. The present study aimed to evaluate the expression profile and biological role of miR-455-5p in colorectal carcinoma. Carcinoma tissues and adjacent tissue specimens from 40 patients with colorectal cancer were randomly collected. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was conducted to detect the expression levels of miR-455-5p in colorectal carcinoma and adjacent normal tissues. The biological effects of miR-455-5p on selected colorectal cancer cells were assessed using bromodeoxyuridine assays, wound healing migration assays and flow cytometry. Bioinformatics analysis was implemented to predict the potential target genes of miR-455-5p in colorectal cancer. The expression levels of target genes were further validated by RT-qPCR and western blot analysis of the mRNA and protein levels. The results of the experiments demonstrated that miR-455-5p expression was downregulated in colorectal cancer tissues compared with adjacent normal tissues. In colorectal cancer cells (SW-480, HT-29 and HCT-116), miR-455-5p was observed to inhibit cell proliferation and migration while promoting cell apoptosis. Bioinformatics analysis predicted that the oncogene phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) was one of the top ranked target genes of miR-455-5p in colorectal cancer cells. This association was validated by RT-qPCR and western blotting. In vivo studies revealed that the expression level of miR-455-5p was significantly downregulated in human colorectal cancer. Further in vitro studies suggested that miR-455-5p may prevent the development of colorectal cancer by downregulating the oncogene PIK3R1. It was concluded that miR-455-5p may target and downregulate PIK3R1 in colorectal cancer. D.A. Spandidos 2019-02 2018-12-21 /pmc/articles/PMC6341642/ /pubmed/30675279 http://dx.doi.org/10.3892/ol.2018.9862 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Jinqiu
Lu, Yang
Zeng, Yiyong
Zhang, Leming
Ke, Kongliang
Guo, Yu
Expression profile and biological function of miR-455-5p in colorectal carcinoma
title Expression profile and biological function of miR-455-5p in colorectal carcinoma
title_full Expression profile and biological function of miR-455-5p in colorectal carcinoma
title_fullStr Expression profile and biological function of miR-455-5p in colorectal carcinoma
title_full_unstemmed Expression profile and biological function of miR-455-5p in colorectal carcinoma
title_short Expression profile and biological function of miR-455-5p in colorectal carcinoma
title_sort expression profile and biological function of mir-455-5p in colorectal carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341642/
https://www.ncbi.nlm.nih.gov/pubmed/30675279
http://dx.doi.org/10.3892/ol.2018.9862
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