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miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5

Previous studies have reported that microRNAs regulate gene expression and transcription. miR-21 have been identified to play a role in many types of cancer. KLF5 functions as a tumor inhibitor in certain cancers. However, the role of KLF5 plays in hepatocellular carcinoma (HCC), especially concerni...

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Autores principales: Wang, Jian, Chu, Yanfeng, Xu, Mei, Zhang, Xiumei, Zhou, Yumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341730/
https://www.ncbi.nlm.nih.gov/pubmed/30675287
http://dx.doi.org/10.3892/ol.2018.9843
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author Wang, Jian
Chu, Yanfeng
Xu, Mei
Zhang, Xiumei
Zhou, Yumei
Xu, Mei
author_facet Wang, Jian
Chu, Yanfeng
Xu, Mei
Zhang, Xiumei
Zhou, Yumei
Xu, Mei
author_sort Wang, Jian
collection PubMed
description Previous studies have reported that microRNAs regulate gene expression and transcription. miR-21 have been identified to play a role in many types of cancer. KLF5 functions as a tumor inhibitor in certain cancers. However, the role of KLF5 plays in hepatocellular carcinoma (HCC), especially concerning the relationship between miR-21 and the KLF5 gene remains to be determined. Reverse transcription-quantitative PCR (RT-qPCR), western blot analysis, as well as luciferase reporter and Transwell assays were used to determine the expression of miR-21 and KLF5 in Huh 7, SK-HEP-1, LO-2, and HCC tissues. In HCC cells and tissues, the upregulation of miR-21 was identified. HCC cell migratory and invasive abilities significantly increased because of miR-21 overexpression. KLF5 expression was inhibited by miR-21 by targeting its 3′-UTR. KLF5 overexpression alleviated the effect induced by miR-21 on the migratory and invasive ability of the Huh 7 cells. The results therefore show that, HCC cell migration and invasion is significantly suppressed by miR-21 via targeting KLF5. The newly identified miR-21/KLF5 axis provides a useful therapeutic biomarker for HCC treatment.
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spelling pubmed-63417302019-01-23 miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5 Wang, Jian Chu, Yanfeng Xu, Mei Zhang, Xiumei Zhou, Yumei Xu, Mei Oncol Lett Articles Previous studies have reported that microRNAs regulate gene expression and transcription. miR-21 have been identified to play a role in many types of cancer. KLF5 functions as a tumor inhibitor in certain cancers. However, the role of KLF5 plays in hepatocellular carcinoma (HCC), especially concerning the relationship between miR-21 and the KLF5 gene remains to be determined. Reverse transcription-quantitative PCR (RT-qPCR), western blot analysis, as well as luciferase reporter and Transwell assays were used to determine the expression of miR-21 and KLF5 in Huh 7, SK-HEP-1, LO-2, and HCC tissues. In HCC cells and tissues, the upregulation of miR-21 was identified. HCC cell migratory and invasive abilities significantly increased because of miR-21 overexpression. KLF5 expression was inhibited by miR-21 by targeting its 3′-UTR. KLF5 overexpression alleviated the effect induced by miR-21 on the migratory and invasive ability of the Huh 7 cells. The results therefore show that, HCC cell migration and invasion is significantly suppressed by miR-21 via targeting KLF5. The newly identified miR-21/KLF5 axis provides a useful therapeutic biomarker for HCC treatment. D.A. Spandidos 2019-02 2018-12-18 /pmc/articles/PMC6341730/ /pubmed/30675287 http://dx.doi.org/10.3892/ol.2018.9843 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Jian
Chu, Yanfeng
Xu, Mei
Zhang, Xiumei
Zhou, Yumei
Xu, Mei
miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title_full miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title_fullStr miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title_full_unstemmed miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title_short miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5
title_sort mir-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting klf5
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6341730/
https://www.ncbi.nlm.nih.gov/pubmed/30675287
http://dx.doi.org/10.3892/ol.2018.9843
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