Cargando…

Effects of extracorporeal shock waves on neuralgia in diabetic rats

OBJECTIVE: The aim of this study was to observe the effects of extracorporeal shock waves (ECSWs) on neuralgia in diabetic rats. MATERIALS AND METHODS: Diabetic neuralgia model was established in rats via injection of streptozotocin. The rats were divided into diabetic neuralgia group (Group A, n=6)...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Yue, Dai, Hong, Long, Juan, Kang, Xin-Guo, He, Chun-Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342217/
https://www.ncbi.nlm.nih.gov/pubmed/30705604
http://dx.doi.org/10.2147/JPR.S177585
_version_ 1783389091993223168
author Zhou, Yue
Dai, Hong
Long, Juan
Kang, Xin-Guo
He, Chun-Jing
author_facet Zhou, Yue
Dai, Hong
Long, Juan
Kang, Xin-Guo
He, Chun-Jing
author_sort Zhou, Yue
collection PubMed
description OBJECTIVE: The aim of this study was to observe the effects of extracorporeal shock waves (ECSWs) on neuralgia in diabetic rats. MATERIALS AND METHODS: Diabetic neuralgia model was established in rats via injection of streptozotocin. The rats were divided into diabetic neuralgia group (Group A, n=6) and ECSW treatment group (Group B, n=6). Another six rats were taken as control group (Group C, n=6). The mechanical withdrawal threshold (MWT) and thermal withdrawal latencies (TWLs) were measured at specific points throughout the experiment, and the sciatic nerve was bluntly severed under anesthesia after the last measurement. The protein expressions of Sphk1 and TNF-α were detected by Western blot, and the mRNA expressions of Sphk1 and TNF-α were detected by reverse transcription PCR. The structure of the sciatic nerve was observed by electron microscopy. RESULTS: Compared with Group C, MWT and TWLs were decreased significantly in Groups A and B (P< 0.05). The protein expressions of TNF-α and Sphk1 in Groups A and B were both significantly higher than those in Group C (P<0.05), with higher expression in Group A than in Group B (P<0.05). The mRNA expressions of TNF-α and Sphk1 were similar. Electron microscopy showed the intact structure of the myelin sheaths of the sciatic nerve fibers in Group C, whereas the structure of the nerve fibers was damaged, with a large number of vacuoles in the myelin sheath in Group A. In Group B, the vacuoles were occasionally formed on the sciatic nerve myelin sheath, with more compact and tidy layer arrangement compared with Group A. CONCLUSION: ECSWs can relieve neuralgia in diabetic rats. Sphk1 and TNF-α may be involved in the occurrence and development of diabetic peripheral neuralgia.
format Online
Article
Text
id pubmed-6342217
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-63422172019-01-31 Effects of extracorporeal shock waves on neuralgia in diabetic rats Zhou, Yue Dai, Hong Long, Juan Kang, Xin-Guo He, Chun-Jing J Pain Res Original Research OBJECTIVE: The aim of this study was to observe the effects of extracorporeal shock waves (ECSWs) on neuralgia in diabetic rats. MATERIALS AND METHODS: Diabetic neuralgia model was established in rats via injection of streptozotocin. The rats were divided into diabetic neuralgia group (Group A, n=6) and ECSW treatment group (Group B, n=6). Another six rats were taken as control group (Group C, n=6). The mechanical withdrawal threshold (MWT) and thermal withdrawal latencies (TWLs) were measured at specific points throughout the experiment, and the sciatic nerve was bluntly severed under anesthesia after the last measurement. The protein expressions of Sphk1 and TNF-α were detected by Western blot, and the mRNA expressions of Sphk1 and TNF-α were detected by reverse transcription PCR. The structure of the sciatic nerve was observed by electron microscopy. RESULTS: Compared with Group C, MWT and TWLs were decreased significantly in Groups A and B (P< 0.05). The protein expressions of TNF-α and Sphk1 in Groups A and B were both significantly higher than those in Group C (P<0.05), with higher expression in Group A than in Group B (P<0.05). The mRNA expressions of TNF-α and Sphk1 were similar. Electron microscopy showed the intact structure of the myelin sheaths of the sciatic nerve fibers in Group C, whereas the structure of the nerve fibers was damaged, with a large number of vacuoles in the myelin sheath in Group A. In Group B, the vacuoles were occasionally formed on the sciatic nerve myelin sheath, with more compact and tidy layer arrangement compared with Group A. CONCLUSION: ECSWs can relieve neuralgia in diabetic rats. Sphk1 and TNF-α may be involved in the occurrence and development of diabetic peripheral neuralgia. Dove Medical Press 2019-01-17 /pmc/articles/PMC6342217/ /pubmed/30705604 http://dx.doi.org/10.2147/JPR.S177585 Text en © 2019 Zhou et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhou, Yue
Dai, Hong
Long, Juan
Kang, Xin-Guo
He, Chun-Jing
Effects of extracorporeal shock waves on neuralgia in diabetic rats
title Effects of extracorporeal shock waves on neuralgia in diabetic rats
title_full Effects of extracorporeal shock waves on neuralgia in diabetic rats
title_fullStr Effects of extracorporeal shock waves on neuralgia in diabetic rats
title_full_unstemmed Effects of extracorporeal shock waves on neuralgia in diabetic rats
title_short Effects of extracorporeal shock waves on neuralgia in diabetic rats
title_sort effects of extracorporeal shock waves on neuralgia in diabetic rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342217/
https://www.ncbi.nlm.nih.gov/pubmed/30705604
http://dx.doi.org/10.2147/JPR.S177585
work_keys_str_mv AT zhouyue effectsofextracorporealshockwavesonneuralgiaindiabeticrats
AT daihong effectsofextracorporealshockwavesonneuralgiaindiabeticrats
AT longjuan effectsofextracorporealshockwavesonneuralgiaindiabeticrats
AT kangxinguo effectsofextracorporealshockwavesonneuralgiaindiabeticrats
AT hechunjing effectsofextracorporealshockwavesonneuralgiaindiabeticrats