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Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis

Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic ba...

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Autores principales: Furci, Leonardo, Jain, Ritushree, Stassen, Joost, Berkowitz, Oliver, Whelan, James, Roquis, David, Baillet, Victoire, Colot, Vincent, Johannes, Frank, Ton, Jurriaan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342528/
https://www.ncbi.nlm.nih.gov/pubmed/30608232
http://dx.doi.org/10.7554/eLife.40655
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author Furci, Leonardo
Jain, Ritushree
Stassen, Joost
Berkowitz, Oliver
Whelan, James
Roquis, David
Baillet, Victoire
Colot, Vincent
Johannes, Frank
Ton, Jurriaan
author_facet Furci, Leonardo
Jain, Ritushree
Stassen, Joost
Berkowitz, Oliver
Whelan, James
Roquis, David
Baillet, Victoire
Colot, Vincent
Johannes, Frank
Ton, Jurriaan
author_sort Furci, Leonardo
collection PubMed
description Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome.
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spelling pubmed-63425282019-01-24 Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis Furci, Leonardo Jain, Ritushree Stassen, Joost Berkowitz, Oliver Whelan, James Roquis, David Baillet, Victoire Colot, Vincent Johannes, Frank Ton, Jurriaan eLife Plant Biology Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome. eLife Sciences Publications, Ltd 2019-01-04 /pmc/articles/PMC6342528/ /pubmed/30608232 http://dx.doi.org/10.7554/eLife.40655 Text en © 2019, Furci et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Furci, Leonardo
Jain, Ritushree
Stassen, Joost
Berkowitz, Oliver
Whelan, James
Roquis, David
Baillet, Victoire
Colot, Vincent
Johannes, Frank
Ton, Jurriaan
Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title_full Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title_fullStr Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title_full_unstemmed Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title_short Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
title_sort identification and characterisation of hypomethylated dna loci controlling quantitative resistance in arabidopsis
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342528/
https://www.ncbi.nlm.nih.gov/pubmed/30608232
http://dx.doi.org/10.7554/eLife.40655
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