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In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study
BACKGROUND: Flow-diverter stents (FDSs) have revolutionised the treatment of intracranial aneurysms. However, associated dual antiplatelet treatment is mandatory. We investigated the biocompatibility of three proprietary antithrombogenic coatings applied to FDSs. METHODS: After Institutional Animal...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342750/ https://www.ncbi.nlm.nih.gov/pubmed/30671686 http://dx.doi.org/10.1186/s41747-018-0084-z |
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author | Martínez Moreno, Rosa Bhogal, Pervinder Lenz-Habijan, Tim Bannewitz, Catrin Siddiqui, Adnan Lylyk, Pedro Hannes, Ralf Monstadt, Hermann Henkes, Hans |
author_facet | Martínez Moreno, Rosa Bhogal, Pervinder Lenz-Habijan, Tim Bannewitz, Catrin Siddiqui, Adnan Lylyk, Pedro Hannes, Ralf Monstadt, Hermann Henkes, Hans |
author_sort | Martínez Moreno, Rosa |
collection | PubMed |
description | BACKGROUND: Flow-diverter stents (FDSs) have revolutionised the treatment of intracranial aneurysms. However, associated dual antiplatelet treatment is mandatory. We investigated the biocompatibility of three proprietary antithrombogenic coatings applied to FDSs. METHODS: After Institutional Animal Care and Use Committee approval, four domestic juvenile female dogs (weight 19.9 ± 0.9 kg, mean ± standard deviation) were commenced on three different oral antiplatelet regimes: no medication (n = 1), acetylsalicylic acid (n = 2), and acetylsalicylic acid and clopidogrel (n = 1). Four p64 FDSs were randomly implanted into the subclavian, common carotid, and external carotid arteries of each dog, including both uncoated p64 stents and p64 stents coated with three different antithrombogenic hydrophilic coating (HPC). Angiography and histological examinations were performed. Wilcoxon/Kruskal-Wallis and ANOVA were used with p value < 0.05 considered as significant. RESULTS: Minimal inflammatory cell infiltration and no device-associated granulomatous cell inflammation were observed. No significant difference in adventitial inflammation (p = 0.522) or neointimal/medial inflammation (p = 0.384) between coated and uncoated stents as well as between the different stent groups regarding endothelial cell loss, surface fibrin/platelet deposition, medial smooth muscle cell loss, or adventitial fibrosis were found. Acute self-limiting thrombus formed on 6/16 implants (37.5%), and all of the thrombi were noted on devices implanted in the common or external carotid artery irrespective of the surface coating. Two of 12 p64 HPC-coated stents (16.7%) and 1/4 uncoated p64 stents (25%) showed severe or complete stenosis at delayed angiography. CONCLUSIONS: In these preliminary in vivo experiments, HPC-coated p64 FDSs appeared to be biocompatible, without acute inflammation. |
format | Online Article Text |
id | pubmed-6342750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-63427502019-02-07 In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study Martínez Moreno, Rosa Bhogal, Pervinder Lenz-Habijan, Tim Bannewitz, Catrin Siddiqui, Adnan Lylyk, Pedro Hannes, Ralf Monstadt, Hermann Henkes, Hans Eur Radiol Exp Original Article BACKGROUND: Flow-diverter stents (FDSs) have revolutionised the treatment of intracranial aneurysms. However, associated dual antiplatelet treatment is mandatory. We investigated the biocompatibility of three proprietary antithrombogenic coatings applied to FDSs. METHODS: After Institutional Animal Care and Use Committee approval, four domestic juvenile female dogs (weight 19.9 ± 0.9 kg, mean ± standard deviation) were commenced on three different oral antiplatelet regimes: no medication (n = 1), acetylsalicylic acid (n = 2), and acetylsalicylic acid and clopidogrel (n = 1). Four p64 FDSs were randomly implanted into the subclavian, common carotid, and external carotid arteries of each dog, including both uncoated p64 stents and p64 stents coated with three different antithrombogenic hydrophilic coating (HPC). Angiography and histological examinations were performed. Wilcoxon/Kruskal-Wallis and ANOVA were used with p value < 0.05 considered as significant. RESULTS: Minimal inflammatory cell infiltration and no device-associated granulomatous cell inflammation were observed. No significant difference in adventitial inflammation (p = 0.522) or neointimal/medial inflammation (p = 0.384) between coated and uncoated stents as well as between the different stent groups regarding endothelial cell loss, surface fibrin/platelet deposition, medial smooth muscle cell loss, or adventitial fibrosis were found. Acute self-limiting thrombus formed on 6/16 implants (37.5%), and all of the thrombi were noted on devices implanted in the common or external carotid artery irrespective of the surface coating. Two of 12 p64 HPC-coated stents (16.7%) and 1/4 uncoated p64 stents (25%) showed severe or complete stenosis at delayed angiography. CONCLUSIONS: In these preliminary in vivo experiments, HPC-coated p64 FDSs appeared to be biocompatible, without acute inflammation. Springer International Publishing 2019-01-22 /pmc/articles/PMC6342750/ /pubmed/30671686 http://dx.doi.org/10.1186/s41747-018-0084-z Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Martínez Moreno, Rosa Bhogal, Pervinder Lenz-Habijan, Tim Bannewitz, Catrin Siddiqui, Adnan Lylyk, Pedro Hannes, Ralf Monstadt, Hermann Henkes, Hans In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title | In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title_full | In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title_fullStr | In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title_full_unstemmed | In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title_short | In vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
title_sort | in vivo canine study of three different coatings applied to p64 flow-diverter stents: initial biocompatibility study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342750/ https://www.ncbi.nlm.nih.gov/pubmed/30671686 http://dx.doi.org/10.1186/s41747-018-0084-z |
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