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Keep moving and stay in a good shape to find your homologous recombination partner
Genomic DNA is constantly exposed to damage. Among the lesion in DNA, double-strand breaks (DSB), because they disrupt the two strands of the DNA double helix, are the more dangerous. DSB are repaired through two evolutionary conserved mechanisms: Non-Homologous End Joining (NHEJ) and Homologous Rec...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342867/ https://www.ncbi.nlm.nih.gov/pubmed/30097675 http://dx.doi.org/10.1007/s00294-018-0873-1 |
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author | Bordelet, Hélène Dubrana, Karine |
author_facet | Bordelet, Hélène Dubrana, Karine |
author_sort | Bordelet, Hélène |
collection | PubMed |
description | Genomic DNA is constantly exposed to damage. Among the lesion in DNA, double-strand breaks (DSB), because they disrupt the two strands of the DNA double helix, are the more dangerous. DSB are repaired through two evolutionary conserved mechanisms: Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Whereas NHEJ simply reseals the double helix with no or minimal processing, HR necessitates the formation of a 3′ssDNA through the processing of DSB ends by the resection machinery and relies on the recognition and pairing of this 3′ssDNA tails with an intact homologous sequence. Despite years of active research on HR, the manner by which the two homologous sequences find each other in the crowded nucleus, and how this modulates HR efficiency, only recently emerges. Here, we review recent advances in our understanding of the factors limiting the search of a homologous sequence during HR. |
format | Online Article Text |
id | pubmed-6342867 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-63428672019-02-06 Keep moving and stay in a good shape to find your homologous recombination partner Bordelet, Hélène Dubrana, Karine Curr Genet Review Genomic DNA is constantly exposed to damage. Among the lesion in DNA, double-strand breaks (DSB), because they disrupt the two strands of the DNA double helix, are the more dangerous. DSB are repaired through two evolutionary conserved mechanisms: Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Whereas NHEJ simply reseals the double helix with no or minimal processing, HR necessitates the formation of a 3′ssDNA through the processing of DSB ends by the resection machinery and relies on the recognition and pairing of this 3′ssDNA tails with an intact homologous sequence. Despite years of active research on HR, the manner by which the two homologous sequences find each other in the crowded nucleus, and how this modulates HR efficiency, only recently emerges. Here, we review recent advances in our understanding of the factors limiting the search of a homologous sequence during HR. Springer Berlin Heidelberg 2018-08-10 2019 /pmc/articles/PMC6342867/ /pubmed/30097675 http://dx.doi.org/10.1007/s00294-018-0873-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Review Bordelet, Hélène Dubrana, Karine Keep moving and stay in a good shape to find your homologous recombination partner |
title | Keep moving and stay in a good shape to find your homologous recombination partner |
title_full | Keep moving and stay in a good shape to find your homologous recombination partner |
title_fullStr | Keep moving and stay in a good shape to find your homologous recombination partner |
title_full_unstemmed | Keep moving and stay in a good shape to find your homologous recombination partner |
title_short | Keep moving and stay in a good shape to find your homologous recombination partner |
title_sort | keep moving and stay in a good shape to find your homologous recombination partner |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342867/ https://www.ncbi.nlm.nih.gov/pubmed/30097675 http://dx.doi.org/10.1007/s00294-018-0873-1 |
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