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Engineering of CRISPR-Cas12b for human genome editing

The type-V CRISPR effector Cas12b (formerly known as C2c1) has been challenging to develop for genome editing in human cells, at least in part due to the high temperature requirement of the characterized family members. Here we explore the diversity of the Cas12b family and identify a promising cand...

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Detalles Bibliográficos
Autores principales:	Strecker, Jonathan, Jones, Sara, Koopal, Balwina, Schmid-Burgk, Jonathan, Zetsche, Bernd, Gao, Linyi, Makarova, Kira S., Koonin, Eugene V., Zhang, Feng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342934/ https://www.ncbi.nlm.nih.gov/pubmed/30670702 http://dx.doi.org/10.1038/s41467-018-08224-4

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