Cargando…
Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion
Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and GFP 11, has previously provided a convenient approach to measure recombinant protein titers in cell supernatants. A...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6342966/ https://www.ncbi.nlm.nih.gov/pubmed/30670736 http://dx.doi.org/10.1038/s41598-018-36559-x |