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Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects

While new biomaterials for regenerative therapies are being reported in the literature, clinical translation is slow. Some existing regenerative approaches rely on high doses of growth factors, such as bone morphogenetic protein‐2 (BMP‐2) in bone regeneration, which can cause serious side effects. A...

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Autores principales: Cheng, Zhe A., Alba‐Perez, Andres, Gonzalez‐Garcia, Cristina, Donnelly, Hannah, Llopis‐Hernandez, Virginia, Jayawarna, Vineetha, Childs, Peter, Shields, David W., Cantini, Marco, Ruiz‐Cantu, Laura, Reid, Andrew, Windmill, James F. C., Addison, Elena S., Corr, Sandra, Marshall, William G., Dalby, Matthew J., Salmeron‐Sanchez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343071/
https://www.ncbi.nlm.nih.gov/pubmed/30693176
http://dx.doi.org/10.1002/advs.201800361
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author Cheng, Zhe A.
Alba‐Perez, Andres
Gonzalez‐Garcia, Cristina
Donnelly, Hannah
Llopis‐Hernandez, Virginia
Jayawarna, Vineetha
Childs, Peter
Shields, David W.
Cantini, Marco
Ruiz‐Cantu, Laura
Reid, Andrew
Windmill, James F. C.
Addison, Elena S.
Corr, Sandra
Marshall, William G.
Dalby, Matthew J.
Salmeron‐Sanchez, Manuel
author_facet Cheng, Zhe A.
Alba‐Perez, Andres
Gonzalez‐Garcia, Cristina
Donnelly, Hannah
Llopis‐Hernandez, Virginia
Jayawarna, Vineetha
Childs, Peter
Shields, David W.
Cantini, Marco
Ruiz‐Cantu, Laura
Reid, Andrew
Windmill, James F. C.
Addison, Elena S.
Corr, Sandra
Marshall, William G.
Dalby, Matthew J.
Salmeron‐Sanchez, Manuel
author_sort Cheng, Zhe A.
collection PubMed
description While new biomaterials for regenerative therapies are being reported in the literature, clinical translation is slow. Some existing regenerative approaches rely on high doses of growth factors, such as bone morphogenetic protein‐2 (BMP‐2) in bone regeneration, which can cause serious side effects. An ultralow‐dose growth factor technology is described yielding high bioactivity based on a simple polymer, poly(ethyl acrylate) (PEA), and mechanisms to drive stem cell differentiation and bone regeneration in a critical‐sized murine defect model with translation to a clinical veterinary setting are reported. This material‐based technology triggers spontaneous fibronectin organization and stimulates growth factor signalling, enabling synergistic integrin and BMP‐2 receptor activation in mesenchymal stem cells. To translate this technology, plasma‐polymerized PEA is used on 2D and 3D substrates to enhance cell signalling in vitro, showing the complete healing of a critical‐sized bone injury in mice in vivo. Efficacy is demonstrated in a Münsterländer dog with a nonhealing humerus fracture, establishing the clinical translation of advanced ultralow‐dose growth factor treatment.
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spelling pubmed-63430712019-01-28 Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects Cheng, Zhe A. Alba‐Perez, Andres Gonzalez‐Garcia, Cristina Donnelly, Hannah Llopis‐Hernandez, Virginia Jayawarna, Vineetha Childs, Peter Shields, David W. Cantini, Marco Ruiz‐Cantu, Laura Reid, Andrew Windmill, James F. C. Addison, Elena S. Corr, Sandra Marshall, William G. Dalby, Matthew J. Salmeron‐Sanchez, Manuel Adv Sci (Weinh) Full Papers While new biomaterials for regenerative therapies are being reported in the literature, clinical translation is slow. Some existing regenerative approaches rely on high doses of growth factors, such as bone morphogenetic protein‐2 (BMP‐2) in bone regeneration, which can cause serious side effects. An ultralow‐dose growth factor technology is described yielding high bioactivity based on a simple polymer, poly(ethyl acrylate) (PEA), and mechanisms to drive stem cell differentiation and bone regeneration in a critical‐sized murine defect model with translation to a clinical veterinary setting are reported. This material‐based technology triggers spontaneous fibronectin organization and stimulates growth factor signalling, enabling synergistic integrin and BMP‐2 receptor activation in mesenchymal stem cells. To translate this technology, plasma‐polymerized PEA is used on 2D and 3D substrates to enhance cell signalling in vitro, showing the complete healing of a critical‐sized bone injury in mice in vivo. Efficacy is demonstrated in a Münsterländer dog with a nonhealing humerus fracture, establishing the clinical translation of advanced ultralow‐dose growth factor treatment. John Wiley and Sons Inc. 2018-11-19 /pmc/articles/PMC6343071/ /pubmed/30693176 http://dx.doi.org/10.1002/advs.201800361 Text en © 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Cheng, Zhe A.
Alba‐Perez, Andres
Gonzalez‐Garcia, Cristina
Donnelly, Hannah
Llopis‐Hernandez, Virginia
Jayawarna, Vineetha
Childs, Peter
Shields, David W.
Cantini, Marco
Ruiz‐Cantu, Laura
Reid, Andrew
Windmill, James F. C.
Addison, Elena S.
Corr, Sandra
Marshall, William G.
Dalby, Matthew J.
Salmeron‐Sanchez, Manuel
Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title_full Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title_fullStr Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title_full_unstemmed Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title_short Nanoscale Coatings for Ultralow Dose BMP‐2‐Driven Regeneration of Critical‐Sized Bone Defects
title_sort nanoscale coatings for ultralow dose bmp‐2‐driven regeneration of critical‐sized bone defects
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343071/
https://www.ncbi.nlm.nih.gov/pubmed/30693176
http://dx.doi.org/10.1002/advs.201800361
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