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Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod

The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens...

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Autores principales: Guyon, Anais, Smith, Kirsty F., Charry, Maria P., Champeau, Olivier, Tremblay, Louis A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343104/
https://www.ncbi.nlm.nih.gov/pubmed/30701062
http://dx.doi.org/10.4081/xeno.2018.7674
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author Guyon, Anais
Smith, Kirsty F.
Charry, Maria P.
Champeau, Olivier
Tremblay, Louis A.
author_facet Guyon, Anais
Smith, Kirsty F.
Charry, Maria P.
Champeau, Olivier
Tremblay, Louis A.
author_sort Guyon, Anais
collection PubMed
description The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens pectinatus. Acute toxicity tests were conducted to determine the sensitivity of G. pectinatus to the chemicals. All chemicals impacted breeding, hatching and egg viability. Diclofenac (1 mg.L(-1)) reduced the number of eggs per gravid female. Benzophenone (0.5 mg.L(-1)) decreased egg hatching success. Exposure to the reference toxicant copper (0.02 mg.L(-1)) led to unsuccessful hatching. Effects on DNA methylation was estimated by the percentage of 5- methylcytosine. The treatments resulted in strong differences in DNA methylation with increased methylation in the exposed animals. The two chemicals impacted both egg viability and the induction of differential DNA methylation, suggesting potential intra- and trans-generational evolutionary effects.
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spelling pubmed-63431042019-01-30 Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod Guyon, Anais Smith, Kirsty F. Charry, Maria P. Champeau, Olivier Tremblay, Louis A. J Xenobiot Brief Report The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens pectinatus. Acute toxicity tests were conducted to determine the sensitivity of G. pectinatus to the chemicals. All chemicals impacted breeding, hatching and egg viability. Diclofenac (1 mg.L(-1)) reduced the number of eggs per gravid female. Benzophenone (0.5 mg.L(-1)) decreased egg hatching success. Exposure to the reference toxicant copper (0.02 mg.L(-1)) led to unsuccessful hatching. Effects on DNA methylation was estimated by the percentage of 5- methylcytosine. The treatments resulted in strong differences in DNA methylation with increased methylation in the exposed animals. The two chemicals impacted both egg viability and the induction of differential DNA methylation, suggesting potential intra- and trans-generational evolutionary effects. PAGEPress Publications, Pavia, Italy 2018-10-29 /pmc/articles/PMC6343104/ /pubmed/30701062 http://dx.doi.org/10.4081/xeno.2018.7674 Text en ©Copyright A. Guyon et al., 2018 http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0).
spellingShingle Brief Report
Guyon, Anais
Smith, Kirsty F.
Charry, Maria P.
Champeau, Olivier
Tremblay, Louis A.
Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title_full Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title_fullStr Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title_full_unstemmed Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title_short Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
title_sort effects of chronic exposure to benzophenone and diclofenac on dna methylation levels and reproductive success in a marine copepod
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343104/
https://www.ncbi.nlm.nih.gov/pubmed/30701062
http://dx.doi.org/10.4081/xeno.2018.7674
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