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Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod
The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343104/ https://www.ncbi.nlm.nih.gov/pubmed/30701062 http://dx.doi.org/10.4081/xeno.2018.7674 |
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author | Guyon, Anais Smith, Kirsty F. Charry, Maria P. Champeau, Olivier Tremblay, Louis A. |
author_facet | Guyon, Anais Smith, Kirsty F. Charry, Maria P. Champeau, Olivier Tremblay, Louis A. |
author_sort | Guyon, Anais |
collection | PubMed |
description | The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens pectinatus. Acute toxicity tests were conducted to determine the sensitivity of G. pectinatus to the chemicals. All chemicals impacted breeding, hatching and egg viability. Diclofenac (1 mg.L(-1)) reduced the number of eggs per gravid female. Benzophenone (0.5 mg.L(-1)) decreased egg hatching success. Exposure to the reference toxicant copper (0.02 mg.L(-1)) led to unsuccessful hatching. Effects on DNA methylation was estimated by the percentage of 5- methylcytosine. The treatments resulted in strong differences in DNA methylation with increased methylation in the exposed animals. The two chemicals impacted both egg viability and the induction of differential DNA methylation, suggesting potential intra- and trans-generational evolutionary effects. |
format | Online Article Text |
id | pubmed-6343104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-63431042019-01-30 Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod Guyon, Anais Smith, Kirsty F. Charry, Maria P. Champeau, Olivier Tremblay, Louis A. J Xenobiot Brief Report The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens pectinatus. Acute toxicity tests were conducted to determine the sensitivity of G. pectinatus to the chemicals. All chemicals impacted breeding, hatching and egg viability. Diclofenac (1 mg.L(-1)) reduced the number of eggs per gravid female. Benzophenone (0.5 mg.L(-1)) decreased egg hatching success. Exposure to the reference toxicant copper (0.02 mg.L(-1)) led to unsuccessful hatching. Effects on DNA methylation was estimated by the percentage of 5- methylcytosine. The treatments resulted in strong differences in DNA methylation with increased methylation in the exposed animals. The two chemicals impacted both egg viability and the induction of differential DNA methylation, suggesting potential intra- and trans-generational evolutionary effects. PAGEPress Publications, Pavia, Italy 2018-10-29 /pmc/articles/PMC6343104/ /pubmed/30701062 http://dx.doi.org/10.4081/xeno.2018.7674 Text en ©Copyright A. Guyon et al., 2018 http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0). |
spellingShingle | Brief Report Guyon, Anais Smith, Kirsty F. Charry, Maria P. Champeau, Olivier Tremblay, Louis A. Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title | Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title_full | Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title_fullStr | Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title_full_unstemmed | Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title_short | Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod |
title_sort | effects of chronic exposure to benzophenone and diclofenac on dna methylation levels and reproductive success in a marine copepod |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343104/ https://www.ncbi.nlm.nih.gov/pubmed/30701062 http://dx.doi.org/10.4081/xeno.2018.7674 |
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