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Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile

T cells in colorectal cancer (CRC) are associated with improved survival. However, checkpoint immunotherapies antagonizing the suppression of these cells are ineffective in the great majority of patients. To better understand the immune cell regulation in CRC, we compared tumor-associated T lymphocy...

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Autores principales: Strasser, Katharina, Birnleitner, Hanna, Beer, Andrea, Pils, Dietmar, Gerner, Marlene C., Schmetterer, Klaus G., Bachleitner-Hofmann, Thomas, Stift, Anton, Bergmann, Michael, Oehler, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343804/
https://www.ncbi.nlm.nih.gov/pubmed/30713795
http://dx.doi.org/10.1080/2162402X.2018.1537693
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author Strasser, Katharina
Birnleitner, Hanna
Beer, Andrea
Pils, Dietmar
Gerner, Marlene C.
Schmetterer, Klaus G.
Bachleitner-Hofmann, Thomas
Stift, Anton
Bergmann, Michael
Oehler, Rudolf
author_facet Strasser, Katharina
Birnleitner, Hanna
Beer, Andrea
Pils, Dietmar
Gerner, Marlene C.
Schmetterer, Klaus G.
Bachleitner-Hofmann, Thomas
Stift, Anton
Bergmann, Michael
Oehler, Rudolf
author_sort Strasser, Katharina
collection PubMed
description T cells in colorectal cancer (CRC) are associated with improved survival. However, checkpoint immunotherapies antagonizing the suppression of these cells are ineffective in the great majority of patients. To better understand the immune cell regulation in CRC, we compared tumor-associated T lymphocytes and macrophages to the immune cell infiltrate of normal mucosa. Human colorectal tumor specimen and tumor-distant normal mucosa tissues of the same patients were collected. Phenotypes and functionality of tissue-derived T cells and macrophages were characterized using immunohistochemistry, RNA in situ hybridization, and multiparameter flow cytometry. CRC contained significantly higher numbers of potentially immunosuppressive CD39 and Helios-expressing regulatory T cells in comparison to normal mucosa. Surprisingly, we found a concomitant increase of pro-inflammatory IFNγ -producing T cells. PD-L1(+) stromal cells were decreased in the tumor tissue. Macrophages in the tumor compared to tumor-distant normal tissue appear to have an altered phenotype, identified by HLA-DR, CD14, CX3CR1, and CD64, and tolerogenic CD206(+) macrophages are quantitatively reduced. The prognostic effect of these observed differences between distant mucosa and tumor tissue on the overall survival was examined using gene expression data of 298 CRC patients. The combined gene expression of increased FOXP3, IFNγ, CD14, and decreased CD206 correlated with a poor prognosis in CRC patients. These data reveal that the CRC microenvironment promotes the coexistence of seemingly antagonistic suppressive and pro-inflammatory immune responses and might provide an explanation why a blockade of the PD1/PD-L1 axis is ineffective in CRC. This should be taken into account when designing novel treatment strategies.
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spelling pubmed-63438042019-02-01 Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile Strasser, Katharina Birnleitner, Hanna Beer, Andrea Pils, Dietmar Gerner, Marlene C. Schmetterer, Klaus G. Bachleitner-Hofmann, Thomas Stift, Anton Bergmann, Michael Oehler, Rudolf Oncoimmunology Original Research T cells in colorectal cancer (CRC) are associated with improved survival. However, checkpoint immunotherapies antagonizing the suppression of these cells are ineffective in the great majority of patients. To better understand the immune cell regulation in CRC, we compared tumor-associated T lymphocytes and macrophages to the immune cell infiltrate of normal mucosa. Human colorectal tumor specimen and tumor-distant normal mucosa tissues of the same patients were collected. Phenotypes and functionality of tissue-derived T cells and macrophages were characterized using immunohistochemistry, RNA in situ hybridization, and multiparameter flow cytometry. CRC contained significantly higher numbers of potentially immunosuppressive CD39 and Helios-expressing regulatory T cells in comparison to normal mucosa. Surprisingly, we found a concomitant increase of pro-inflammatory IFNγ -producing T cells. PD-L1(+) stromal cells were decreased in the tumor tissue. Macrophages in the tumor compared to tumor-distant normal tissue appear to have an altered phenotype, identified by HLA-DR, CD14, CX3CR1, and CD64, and tolerogenic CD206(+) macrophages are quantitatively reduced. The prognostic effect of these observed differences between distant mucosa and tumor tissue on the overall survival was examined using gene expression data of 298 CRC patients. The combined gene expression of increased FOXP3, IFNγ, CD14, and decreased CD206 correlated with a poor prognosis in CRC patients. These data reveal that the CRC microenvironment promotes the coexistence of seemingly antagonistic suppressive and pro-inflammatory immune responses and might provide an explanation why a blockade of the PD1/PD-L1 axis is ineffective in CRC. This should be taken into account when designing novel treatment strategies. Taylor & Francis 2018-11-05 /pmc/articles/PMC6343804/ /pubmed/30713795 http://dx.doi.org/10.1080/2162402X.2018.1537693 Text en © 2018 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Original Research
Strasser, Katharina
Birnleitner, Hanna
Beer, Andrea
Pils, Dietmar
Gerner, Marlene C.
Schmetterer, Klaus G.
Bachleitner-Hofmann, Thomas
Stift, Anton
Bergmann, Michael
Oehler, Rudolf
Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title_full Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title_fullStr Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title_full_unstemmed Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title_short Immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
title_sort immunological differences between colorectal cancer and normal mucosa uncover a prognostically relevant immune cell profile
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343804/
https://www.ncbi.nlm.nih.gov/pubmed/30713795
http://dx.doi.org/10.1080/2162402X.2018.1537693
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