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Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers

Understanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well–known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most...

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Autores principales: Yadav, Manoj Kumar, Aravindan, S., Ngangkham, Umakanta, Raghu, S., Prabhukarthikeyan, S. R., Keerthana, U., Marndi, B. C., Adak, Totan, Munda, Susmita, Deshmukh, Rupesh, Pramesh, D., Samantaray, Sanghamitra, Rath, P. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343911/
https://www.ncbi.nlm.nih.gov/pubmed/30673751
http://dx.doi.org/10.1371/journal.pone.0211061
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author Yadav, Manoj Kumar
Aravindan, S.
Ngangkham, Umakanta
Raghu, S.
Prabhukarthikeyan, S. R.
Keerthana, U.
Marndi, B. C.
Adak, Totan
Munda, Susmita
Deshmukh, Rupesh
Pramesh, D.
Samantaray, Sanghamitra
Rath, P. C.
author_facet Yadav, Manoj Kumar
Aravindan, S.
Ngangkham, Umakanta
Raghu, S.
Prabhukarthikeyan, S. R.
Keerthana, U.
Marndi, B. C.
Adak, Totan
Munda, Susmita
Deshmukh, Rupesh
Pramesh, D.
Samantaray, Sanghamitra
Rath, P. C.
author_sort Yadav, Manoj Kumar
collection PubMed
description Understanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well–known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most significant blast resistance gene loci using twenty-eight gene specific markers were investigated in landraces originated from nine diverse rice ecologies of India. Based on phenotypic evaluation, landraces were classified into three distinct groups: highly resistant (21), moderately resistant (70) and susceptible (70). The landraces harbour a range of five to nineteen genes representing blast resistance allele with the frequency varied from 4.96% to 100%. The cluster analysis grouped entire 161 landraces into two major groups. Population structure along with other parameters was also analyzed to understand the evolution of blast resistance gene in rice. The population structure analysis and principal coordinate analysis classified the landraces into two sub–populations. Analysis of molecular variance showed maximum (93%) diversity within the population and least (7%) between populations. Five markers viz; K3957, Pikh, Pi2–i, RM212and RM302 were strongly associated with blast disease with the phenotypic variance of 1.4% to 7.6%. These resistant landraces will serve as a valuable genetic resource for future genomic studies, host–pathogen interaction, identification of novel R genes and rice improvement strategies.
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spelling pubmed-63439112019-02-02 Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers Yadav, Manoj Kumar Aravindan, S. Ngangkham, Umakanta Raghu, S. Prabhukarthikeyan, S. R. Keerthana, U. Marndi, B. C. Adak, Totan Munda, Susmita Deshmukh, Rupesh Pramesh, D. Samantaray, Sanghamitra Rath, P. C. PLoS One Research Article Understanding of genetic diversity is important to explore existing gene in any crop breeding program. Most of the diversity preserved in the landraces which are well–known reservoirs of important traits for biotic and abiotic stresses. In the present study, the genetic diversity at twenty-four most significant blast resistance gene loci using twenty-eight gene specific markers were investigated in landraces originated from nine diverse rice ecologies of India. Based on phenotypic evaluation, landraces were classified into three distinct groups: highly resistant (21), moderately resistant (70) and susceptible (70). The landraces harbour a range of five to nineteen genes representing blast resistance allele with the frequency varied from 4.96% to 100%. The cluster analysis grouped entire 161 landraces into two major groups. Population structure along with other parameters was also analyzed to understand the evolution of blast resistance gene in rice. The population structure analysis and principal coordinate analysis classified the landraces into two sub–populations. Analysis of molecular variance showed maximum (93%) diversity within the population and least (7%) between populations. Five markers viz; K3957, Pikh, Pi2–i, RM212and RM302 were strongly associated with blast disease with the phenotypic variance of 1.4% to 7.6%. These resistant landraces will serve as a valuable genetic resource for future genomic studies, host–pathogen interaction, identification of novel R genes and rice improvement strategies. Public Library of Science 2019-01-23 /pmc/articles/PMC6343911/ /pubmed/30673751 http://dx.doi.org/10.1371/journal.pone.0211061 Text en © 2019 Yadav et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yadav, Manoj Kumar
Aravindan, S.
Ngangkham, Umakanta
Raghu, S.
Prabhukarthikeyan, S. R.
Keerthana, U.
Marndi, B. C.
Adak, Totan
Munda, Susmita
Deshmukh, Rupesh
Pramesh, D.
Samantaray, Sanghamitra
Rath, P. C.
Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title_full Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title_fullStr Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title_full_unstemmed Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title_short Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers
title_sort blast resistance in indian rice landraces: genetic dissection by gene specific markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343911/
https://www.ncbi.nlm.nih.gov/pubmed/30673751
http://dx.doi.org/10.1371/journal.pone.0211061
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