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Analysis of Manifestation of CC and CXC Chemokine Genes in Olive Flounders (Paralichthys olivaceus) Artificially Infected with VHSV during the Early Developmental Stage
Chemokines is a small protein that plays a major role in inflammatory reactions and viral infections as a chemotactic factor of cytokines involved in innate immunity. Most of the chemokines belong to the chemokine groups CC and CXC. To investigate the immune system of the olive flounder (Paralichthy...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society of Developmental Biology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344357/ https://www.ncbi.nlm.nih.gov/pubmed/30680333 http://dx.doi.org/10.12717/DR.2018.22.4.341 |
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author | Kim, Kyung-Hee Kim, Woo-Jin Park, Choul-Ji Park, Jong-Won Noh, Gyeong Eon Lee, Seunghyung Lee, Young Mee Kim, Hyun Chul |
author_facet | Kim, Kyung-Hee Kim, Woo-Jin Park, Choul-Ji Park, Jong-Won Noh, Gyeong Eon Lee, Seunghyung Lee, Young Mee Kim, Hyun Chul |
author_sort | Kim, Kyung-Hee |
collection | PubMed |
description | Chemokines is a small protein that plays a major role in inflammatory reactions and viral infections as a chemotactic factor of cytokines involved in innate immunity. Most of the chemokines belong to the chemokine groups CC and CXC. To investigate the immune system of the olive flounder (Paralichthys olivaceus), an expression pattern specifically induced in the early developmental stages of analysis is examined using qRT-PCR. We also examined tissue-specific expression of both CC and CXC chemokine in healthy olive flounder samples. CC and CXC chemokine shows increased expression after immune-related organs are formed compared to expression during early development. CC chemokine was more highly expressed in the fin, but CXC chemokine showed higher expression in the gills, spleen, intestines, and stomach. Spatial and temporal expression analysis of CC and CXC chemokine were performed following viral hemorrhagic septicemia virus (VHSV) infection. CC chemokine showed high expression in the gills, which are respiratory organs, whereas CXC chemokine was more highly expressed in the kidneys, an immune-related organ. These results suggest that CC and CXC chemokine play an important role in the immune response of the olive flounder, and may be used as basic data for the immunological activity and gene analysis of it as well as other fish. |
format | Online Article Text |
id | pubmed-6344357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-63443572019-01-24 Analysis of Manifestation of CC and CXC Chemokine Genes in Olive Flounders (Paralichthys olivaceus) Artificially Infected with VHSV during the Early Developmental Stage Kim, Kyung-Hee Kim, Woo-Jin Park, Choul-Ji Park, Jong-Won Noh, Gyeong Eon Lee, Seunghyung Lee, Young Mee Kim, Hyun Chul Dev Reprod Original Research Paper Chemokines is a small protein that plays a major role in inflammatory reactions and viral infections as a chemotactic factor of cytokines involved in innate immunity. Most of the chemokines belong to the chemokine groups CC and CXC. To investigate the immune system of the olive flounder (Paralichthys olivaceus), an expression pattern specifically induced in the early developmental stages of analysis is examined using qRT-PCR. We also examined tissue-specific expression of both CC and CXC chemokine in healthy olive flounder samples. CC and CXC chemokine shows increased expression after immune-related organs are formed compared to expression during early development. CC chemokine was more highly expressed in the fin, but CXC chemokine showed higher expression in the gills, spleen, intestines, and stomach. Spatial and temporal expression analysis of CC and CXC chemokine were performed following viral hemorrhagic septicemia virus (VHSV) infection. CC chemokine showed high expression in the gills, which are respiratory organs, whereas CXC chemokine was more highly expressed in the kidneys, an immune-related organ. These results suggest that CC and CXC chemokine play an important role in the immune response of the olive flounder, and may be used as basic data for the immunological activity and gene analysis of it as well as other fish. Korean Society of Developmental Biology 2018-12 2018-12-31 /pmc/articles/PMC6344357/ /pubmed/30680333 http://dx.doi.org/10.12717/DR.2018.22.4.341 Text en © Copyright 2018 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Kim, Kyung-Hee Kim, Woo-Jin Park, Choul-Ji Park, Jong-Won Noh, Gyeong Eon Lee, Seunghyung Lee, Young Mee Kim, Hyun Chul Analysis of Manifestation of CC and CXC Chemokine Genes in Olive Flounders (Paralichthys olivaceus) Artificially Infected with VHSV during the Early Developmental Stage |
title | Analysis of Manifestation of CC and CXC Chemokine Genes in Olive
Flounders (Paralichthys olivaceus) Artificially Infected with
VHSV during the Early Developmental Stage |
title_full | Analysis of Manifestation of CC and CXC Chemokine Genes in Olive
Flounders (Paralichthys olivaceus) Artificially Infected with
VHSV during the Early Developmental Stage |
title_fullStr | Analysis of Manifestation of CC and CXC Chemokine Genes in Olive
Flounders (Paralichthys olivaceus) Artificially Infected with
VHSV during the Early Developmental Stage |
title_full_unstemmed | Analysis of Manifestation of CC and CXC Chemokine Genes in Olive
Flounders (Paralichthys olivaceus) Artificially Infected with
VHSV during the Early Developmental Stage |
title_short | Analysis of Manifestation of CC and CXC Chemokine Genes in Olive
Flounders (Paralichthys olivaceus) Artificially Infected with
VHSV during the Early Developmental Stage |
title_sort | analysis of manifestation of cc and cxc chemokine genes in olive
flounders (paralichthys olivaceus) artificially infected with
vhsv during the early developmental stage |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344357/ https://www.ncbi.nlm.nih.gov/pubmed/30680333 http://dx.doi.org/10.12717/DR.2018.22.4.341 |
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