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Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense

Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-...

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Autores principales: Liu, Dongxin, He, Wencong, Jiang, Mingxia, Zhao, Bing, Ou, Xichao, Liu, Chunfa, Xia, Hui, Zhou, Yang, Wang, Shengfen, Song, Yuanyuan, Zheng, Yang, Chen, Qian, Fan, Jiale, He, Guangxue, Zhao, Yanlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344564/
https://www.ncbi.nlm.nih.gov/pubmed/30673881
http://dx.doi.org/10.1186/s13568-019-0734-4
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author Liu, Dongxin
He, Wencong
Jiang, Mingxia
Zhao, Bing
Ou, Xichao
Liu, Chunfa
Xia, Hui
Zhou, Yang
Wang, Shengfen
Song, Yuanyuan
Zheng, Yang
Chen, Qian
Fan, Jiale
He, Guangxue
Zhao, Yanlin
author_facet Liu, Dongxin
He, Wencong
Jiang, Mingxia
Zhao, Bing
Ou, Xichao
Liu, Chunfa
Xia, Hui
Zhou, Yang
Wang, Shengfen
Song, Yuanyuan
Zheng, Yang
Chen, Qian
Fan, Jiale
He, Guangxue
Zhao, Yanlin
author_sort Liu, Dongxin
collection PubMed
description Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-019-0734-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-63445642019-02-08 Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense Liu, Dongxin He, Wencong Jiang, Mingxia Zhao, Bing Ou, Xichao Liu, Chunfa Xia, Hui Zhou, Yang Wang, Shengfen Song, Yuanyuan Zheng, Yang Chen, Qian Fan, Jiale He, Guangxue Zhao, Yanlin AMB Express Original Article Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-019-0734-4) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-01-23 /pmc/articles/PMC6344564/ /pubmed/30673881 http://dx.doi.org/10.1186/s13568-019-0734-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Liu, Dongxin
He, Wencong
Jiang, Mingxia
Zhao, Bing
Ou, Xichao
Liu, Chunfa
Xia, Hui
Zhou, Yang
Wang, Shengfen
Song, Yuanyuan
Zheng, Yang
Chen, Qian
Fan, Jiale
He, Guangxue
Zhao, Yanlin
Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title_full Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title_fullStr Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title_full_unstemmed Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title_short Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense
title_sort development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of mycobacterium abscessus and mycobacterium massiliense
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344564/
https://www.ncbi.nlm.nih.gov/pubmed/30673881
http://dx.doi.org/10.1186/s13568-019-0734-4
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