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Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor
Early growth response 1 (EGR1) is a transcription factor and regulates cellular processes such as proliferation, differentiation, and apoptosis. The expression of EGR1 is rapidly induced in response to several stimuli, and it activates the expression of downstream target genes involved in signaling...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344568/ https://www.ncbi.nlm.nih.gov/pubmed/30674949 http://dx.doi.org/10.1038/s41598-018-36753-x |
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author | Sekiya, Takeshi Kato, Kohsuke Kawaguchi, Atsushi Nagata, Kyosuke |
author_facet | Sekiya, Takeshi Kato, Kohsuke Kawaguchi, Atsushi Nagata, Kyosuke |
author_sort | Sekiya, Takeshi |
collection | PubMed |
description | Early growth response 1 (EGR1) is a transcription factor and regulates cellular processes such as proliferation, differentiation, and apoptosis. The expression of EGR1 is rapidly induced in response to several stimuli, and it activates the expression of downstream target genes involved in signaling cascades. EGR1 gene is also known to be transcribed in early G1 phase. However, the regulation of EGR1 transcription in early G1 phase is not clarified well. Here we found that CCCTC-binding factor (CTCF), a chromatin binding protein, is required to transcribe EGR1 gene at the onset of early G1 phase. We found that CTCF mediated the formation of higher-order chromatin structures among CTCF binding sites located in the EGR1 locus. Disruption of the CTCF-dependent higher-order chromatin structure using nuclease-dead Cas9 (dCas9)-mediated interference reduced the EGR1 transcription in early G1 phase. Collectively, we propose that CTCF has functional roles for the temporal expression of EGR1 in early G1 phase through regulation of higher-order chromatin structure organization. |
format | Online Article Text |
id | pubmed-6344568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63445682019-01-28 Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor Sekiya, Takeshi Kato, Kohsuke Kawaguchi, Atsushi Nagata, Kyosuke Sci Rep Article Early growth response 1 (EGR1) is a transcription factor and regulates cellular processes such as proliferation, differentiation, and apoptosis. The expression of EGR1 is rapidly induced in response to several stimuli, and it activates the expression of downstream target genes involved in signaling cascades. EGR1 gene is also known to be transcribed in early G1 phase. However, the regulation of EGR1 transcription in early G1 phase is not clarified well. Here we found that CCCTC-binding factor (CTCF), a chromatin binding protein, is required to transcribe EGR1 gene at the onset of early G1 phase. We found that CTCF mediated the formation of higher-order chromatin structures among CTCF binding sites located in the EGR1 locus. Disruption of the CTCF-dependent higher-order chromatin structure using nuclease-dead Cas9 (dCas9)-mediated interference reduced the EGR1 transcription in early G1 phase. Collectively, we propose that CTCF has functional roles for the temporal expression of EGR1 in early G1 phase through regulation of higher-order chromatin structure organization. Nature Publishing Group UK 2019-01-23 /pmc/articles/PMC6344568/ /pubmed/30674949 http://dx.doi.org/10.1038/s41598-018-36753-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sekiya, Takeshi Kato, Kohsuke Kawaguchi, Atsushi Nagata, Kyosuke Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title | Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title_full | Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title_fullStr | Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title_full_unstemmed | Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title_short | Involvement of CTCF in transcription regulation of EGR1 at early G1 phase as an architecture factor |
title_sort | involvement of ctcf in transcription regulation of egr1 at early g1 phase as an architecture factor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344568/ https://www.ncbi.nlm.nih.gov/pubmed/30674949 http://dx.doi.org/10.1038/s41598-018-36753-x |
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