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An improved yeast surface display platform for the screening of nanobody immune libraries
Fusions to the C-terminal end of the Aga2p mating adhesion of Saccharomyces cerevisiae have been used in many studies for the selection of affinity reagents by yeast display followed by flow cytometric analysis. Here we present an improved yeast display system for the screening of Nanobody immune li...
Autores principales: | Uchański, Tomasz, Zögg, Thomas, Yin, Jie, Yuan, Daopeng, Wohlkönig, Alexandre, Fischer, Baptiste, Rosenbaum, Daniel M., Kobilka, Brian K., Pardon, Els, Steyaert, Jan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344588/ https://www.ncbi.nlm.nih.gov/pubmed/30674983 http://dx.doi.org/10.1038/s41598-018-37212-3 |
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