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Generation of Markerless Deletions in the Nosocomial Pathogen Clostridium difficile by Induction of DNA Double-Strand Breaks

Clostridium difficile is an important nosocomial pathogen associated with potentially fatal disease induced by the use of antibiotics. Genetic characterization of such clinically important bacteria is often hampered by lack of availability of suitable tools. Here, we describe the use of I-SceI to in...

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Detalles Bibliográficos
Autores principales:	Theophilou, Elena-Stella, Vohra, Prerna, Gallagher, Maurice P., Poxton, Ian R., Blakely, Garry W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2019
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344619/ https://www.ncbi.nlm.nih.gov/pubmed/30478235 http://dx.doi.org/10.1128/AEM.02055-18

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