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Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction
INTRODUCTION: In this fast era of numerous unwanted disasters and because of the severely devastated and degenerated body remains, personal identification of unknown remains has become the most difficult and challenging task. In such instances, dental pulp plays a vital role in identification throug...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344793/ https://www.ncbi.nlm.nih.gov/pubmed/30745788 http://dx.doi.org/10.4103/jfo.jfds_67_16 |
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author | Pawar, Ruchi Kishor More, Chandramani B. |
author_facet | Pawar, Ruchi Kishor More, Chandramani B. |
author_sort | Pawar, Ruchi Kishor |
collection | PubMed |
description | INTRODUCTION: In this fast era of numerous unwanted disasters and because of the severely devastated and degenerated body remains, personal identification of unknown remains has become the most difficult and challenging task. In such instances, dental pulp plays a vital role in identification through deoxyribonucleic acid (DNA). AIM: The aim of the study is to determine sex from tooth pulp tissues by DNA analysis using polymerase chain reaction amplification method under different environmental conditions. MATERIALS AND METHODS: The human extracted teeth were exposed to different conditions such as heat, soil, and open environment. The DNA was extracted from all these teeth including freshly extracted teeth, then quantified, and further amplified with male and female primers. RESULTS: Quantity of DNA content achieved ranged from 5.21 to 62.87 ng/μl. The accuracy in determining sex from pulp DNA ranged from 92% to 100% in the study groups, except from the teeth exposed to uncontrolled heat, as the pulp tissue was burnt completely. The intergroup analysis was statistically highly significant (P < 0.001). Gender determination using the quantity of DNA was found to be nonsignificant (P > 0.05). CONCLUSION: The dental pulp is the reliable source for sex determination in the humid or dry environment compared to uncontrolled heat. |
format | Online Article Text |
id | pubmed-6344793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-63447932019-02-11 Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction Pawar, Ruchi Kishor More, Chandramani B. J Forensic Dent Sci Original Article INTRODUCTION: In this fast era of numerous unwanted disasters and because of the severely devastated and degenerated body remains, personal identification of unknown remains has become the most difficult and challenging task. In such instances, dental pulp plays a vital role in identification through deoxyribonucleic acid (DNA). AIM: The aim of the study is to determine sex from tooth pulp tissues by DNA analysis using polymerase chain reaction amplification method under different environmental conditions. MATERIALS AND METHODS: The human extracted teeth were exposed to different conditions such as heat, soil, and open environment. The DNA was extracted from all these teeth including freshly extracted teeth, then quantified, and further amplified with male and female primers. RESULTS: Quantity of DNA content achieved ranged from 5.21 to 62.87 ng/μl. The accuracy in determining sex from pulp DNA ranged from 92% to 100% in the study groups, except from the teeth exposed to uncontrolled heat, as the pulp tissue was burnt completely. The intergroup analysis was statistically highly significant (P < 0.001). Gender determination using the quantity of DNA was found to be nonsignificant (P > 0.05). CONCLUSION: The dental pulp is the reliable source for sex determination in the humid or dry environment compared to uncontrolled heat. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6344793/ /pubmed/30745788 http://dx.doi.org/10.4103/jfo.jfds_67_16 Text en Copyright: © 2019 Journal of Forensic Dental Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Pawar, Ruchi Kishor More, Chandramani B. Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title | Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title_full | Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title_fullStr | Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title_full_unstemmed | Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title_short | Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
title_sort | sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344793/ https://www.ncbi.nlm.nih.gov/pubmed/30745788 http://dx.doi.org/10.4103/jfo.jfds_67_16 |
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