A new full-length circular DNA sequencing method for viral-sized genomes reveals that RNAi transgenic plants provoke a shift in geminivirus populations in the field

We present a new method, CIDER-Seq (Circular DNA Enrichment sequencing) for the unbiased enrichment and long-read sequencing of viral-sized circular DNA molecules. We used CIDER-Seq to produce single-read full-length virus genomes for the first time. CIDER-Seq combines PCR-free virus enrichment with...

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Detalles Bibliográficos
Autores principales: Mehta, Devang, Hirsch-Hoffmann, Matthias, Were, Mariam, Patrignani, Andrea, Zaidi, Syed Shan-e-Ali, Were, Hassan, Gruissem, Wilhelm, Vanderschuren, Hervé
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344846/
https://www.ncbi.nlm.nih.gov/pubmed/30357413
http://dx.doi.org/10.1093/nar/gky914

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