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AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344853/ https://www.ncbi.nlm.nih.gov/pubmed/30508117 http://dx.doi.org/10.1093/nar/gky1205 |
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author | Jin, Xiaohuan Lv, Zhengyi Gao, Junbao Zhang, Rui Zheng, Ting Yin, Ping Li, Dongqin Peng, Liangcai Cao, Xintao Qin, Yan Persson, Staffan Zheng, Bo Chen, Peng |
author_facet | Jin, Xiaohuan Lv, Zhengyi Gao, Junbao Zhang, Rui Zheng, Ting Yin, Ping Li, Dongqin Peng, Liangcai Cao, Xintao Qin, Yan Persson, Staffan Zheng, Bo Chen, Peng |
author_sort | Jin, Xiaohuan |
collection | PubMed |
description | Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m(1)G and m(1)I methylation was confirmed by mutant analysis, yeast complementation, m(1)G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m(1)G and m(1)I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants. |
format | Online Article Text |
id | pubmed-6344853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-63448532019-01-29 AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana Jin, Xiaohuan Lv, Zhengyi Gao, Junbao Zhang, Rui Zheng, Ting Yin, Ping Li, Dongqin Peng, Liangcai Cao, Xintao Qin, Yan Persson, Staffan Zheng, Bo Chen, Peng Nucleic Acids Res Nucleic Acid Enzymes Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m(1)G and m(1)I methylation was confirmed by mutant analysis, yeast complementation, m(1)G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m(1)G and m(1)I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants. Oxford University Press 2019-01-25 2018-12-01 /pmc/articles/PMC6344853/ /pubmed/30508117 http://dx.doi.org/10.1093/nar/gky1205 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Nucleic Acid Enzymes Jin, Xiaohuan Lv, Zhengyi Gao, Junbao Zhang, Rui Zheng, Ting Yin, Ping Li, Dongqin Peng, Liangcai Cao, Xintao Qin, Yan Persson, Staffan Zheng, Bo Chen, Peng AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title | AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title_full | AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title_fullStr | AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title_full_unstemmed | AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title_short | AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana |
title_sort | attrm5a catalyses 1-methylguanosine and 1-methylinosine formation on trnas and is important for vegetative and reproductive growth in arabidopsis thaliana |
topic | Nucleic Acid Enzymes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344853/ https://www.ncbi.nlm.nih.gov/pubmed/30508117 http://dx.doi.org/10.1093/nar/gky1205 |
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