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AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana

Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to...

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Autores principales: Jin, Xiaohuan, Lv, Zhengyi, Gao, Junbao, Zhang, Rui, Zheng, Ting, Yin, Ping, Li, Dongqin, Peng, Liangcai, Cao, Xintao, Qin, Yan, Persson, Staffan, Zheng, Bo, Chen, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344853/
https://www.ncbi.nlm.nih.gov/pubmed/30508117
http://dx.doi.org/10.1093/nar/gky1205
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author Jin, Xiaohuan
Lv, Zhengyi
Gao, Junbao
Zhang, Rui
Zheng, Ting
Yin, Ping
Li, Dongqin
Peng, Liangcai
Cao, Xintao
Qin, Yan
Persson, Staffan
Zheng, Bo
Chen, Peng
author_facet Jin, Xiaohuan
Lv, Zhengyi
Gao, Junbao
Zhang, Rui
Zheng, Ting
Yin, Ping
Li, Dongqin
Peng, Liangcai
Cao, Xintao
Qin, Yan
Persson, Staffan
Zheng, Bo
Chen, Peng
author_sort Jin, Xiaohuan
collection PubMed
description Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m(1)G and m(1)I methylation was confirmed by mutant analysis, yeast complementation, m(1)G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m(1)G and m(1)I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants.
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spelling pubmed-63448532019-01-29 AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana Jin, Xiaohuan Lv, Zhengyi Gao, Junbao Zhang, Rui Zheng, Ting Yin, Ping Li, Dongqin Peng, Liangcai Cao, Xintao Qin, Yan Persson, Staffan Zheng, Bo Chen, Peng Nucleic Acids Res Nucleic Acid Enzymes Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m(1)G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m(1)G and m(1)I methylation was confirmed by mutant analysis, yeast complementation, m(1)G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m(1)G and m(1)I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants. Oxford University Press 2019-01-25 2018-12-01 /pmc/articles/PMC6344853/ /pubmed/30508117 http://dx.doi.org/10.1093/nar/gky1205 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nucleic Acid Enzymes
Jin, Xiaohuan
Lv, Zhengyi
Gao, Junbao
Zhang, Rui
Zheng, Ting
Yin, Ping
Li, Dongqin
Peng, Liangcai
Cao, Xintao
Qin, Yan
Persson, Staffan
Zheng, Bo
Chen, Peng
AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title_full AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title_fullStr AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title_full_unstemmed AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title_short AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
title_sort attrm5a catalyses 1-methylguanosine and 1-methylinosine formation on trnas and is important for vegetative and reproductive growth in arabidopsis thaliana
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6344853/
https://www.ncbi.nlm.nih.gov/pubmed/30508117
http://dx.doi.org/10.1093/nar/gky1205
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