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Guide RNAs with embedded barcodes boost CRISPR-pooled screens

We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in their loop regions. Our iBAR approach outperforms the conventional method by producing screening results with much lower false-positive and false-negative rates especially with a high multiplicity of infec...

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Detalles Bibliográficos
Autores principales:	Zhu, Shiyou, Cao, Zhongzheng, Liu, Zhiheng, He, Yuan, Wang, Yinan, Yuan, Pengfei, Li, Wei, Tian, Feng, Bao, Ying, Wei, Wensheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345036/ https://www.ncbi.nlm.nih.gov/pubmed/30678704 http://dx.doi.org/10.1186/s13059-019-1628-0

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