Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging

Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents....

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Detalles Bibliográficos
Autores principales: Strauss, Sebastian, Nickels, Philipp C., Strauss, Maximilian T., Sabinina, Vilma J., Ellenberg, Jan, Carter, Jeffrey D., Gupta, Shashi, Janjic, Nebojsa, Jungmann, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345375/
https://www.ncbi.nlm.nih.gov/pubmed/30127504
http://dx.doi.org/10.1038/s41592-018-0105-0
Descripción
Sumario:Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents. Here, we introduce SOMAmers as labeling reagents for DNA-PAINT. We demonstrate the achievable resolution and specificity by labeling and imaging of transmembrane as well as intracellular targets in fixed and live cell-specimen.

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