Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging

Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents....

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Autores principales: Strauss, Sebastian, Nickels, Philipp C., Strauss, Maximilian T., Sabinina, Vilma J., Ellenberg, Jan, Carter, Jeffrey D., Gupta, Shashi, Janjic, Nebojsa, Jungmann, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345375/
https://www.ncbi.nlm.nih.gov/pubmed/30127504
http://dx.doi.org/10.1038/s41592-018-0105-0
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author Strauss, Sebastian
Nickels, Philipp C.
Strauss, Maximilian T.
Sabinina, Vilma J.
Ellenberg, Jan
Carter, Jeffrey D.
Gupta, Shashi
Janjic, Nebojsa
Jungmann, Ralf
author_facet Strauss, Sebastian
Nickels, Philipp C.
Strauss, Maximilian T.
Sabinina, Vilma J.
Ellenberg, Jan
Carter, Jeffrey D.
Gupta, Shashi
Janjic, Nebojsa
Jungmann, Ralf
author_sort Strauss, Sebastian
collection PubMed
description Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents. Here, we introduce SOMAmers as labeling reagents for DNA-PAINT. We demonstrate the achievable resolution and specificity by labeling and imaging of transmembrane as well as intracellular targets in fixed and live cell-specimen.
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spelling pubmed-63453752019-02-20 Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging Strauss, Sebastian Nickels, Philipp C. Strauss, Maximilian T. Sabinina, Vilma J. Ellenberg, Jan Carter, Jeffrey D. Gupta, Shashi Janjic, Nebojsa Jungmann, Ralf Nat Methods Article Super-resolution methods allow the visualization of phenomena thus far elusive to light microscopy. While current implementations are technically approaching true molecular-scale resolution, this does not translate to imaging biological specimen, due to the unavailability of small affinity reagents. Here, we introduce SOMAmers as labeling reagents for DNA-PAINT. We demonstrate the achievable resolution and specificity by labeling and imaging of transmembrane as well as intracellular targets in fixed and live cell-specimen. 2018-08-20 2018-09 /pmc/articles/PMC6345375/ /pubmed/30127504 http://dx.doi.org/10.1038/s41592-018-0105-0 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Strauss, Sebastian
Nickels, Philipp C.
Strauss, Maximilian T.
Sabinina, Vilma J.
Ellenberg, Jan
Carter, Jeffrey D.
Gupta, Shashi
Janjic, Nebojsa
Jungmann, Ralf
Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title_full Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title_fullStr Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title_full_unstemmed Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title_short Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging
title_sort modified aptamers enable quantitative sub-10-nm cellular dna-paint imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345375/
https://www.ncbi.nlm.nih.gov/pubmed/30127504
http://dx.doi.org/10.1038/s41592-018-0105-0
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