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Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia

Preeclampsia is a pregnancy-related hypertensive disorder accounting for 14% of global maternal deaths annually. Preeclampsia — maternal hypertension and proteinuria — is promoted by placental ischemia resulting from reduced uteroplacental perfusion. Here, we assess longitudinal changes in placental...

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Autores principales: Lawrence, Dylan J., Escott, Megan E., Myers, Leann, Intapad, Suttira, Lindsey, Sarah H., Bayer, Carolyn L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345947/
https://www.ncbi.nlm.nih.gov/pubmed/30679723
http://dx.doi.org/10.1038/s41598-018-37310-2
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author Lawrence, Dylan J.
Escott, Megan E.
Myers, Leann
Intapad, Suttira
Lindsey, Sarah H.
Bayer, Carolyn L.
author_facet Lawrence, Dylan J.
Escott, Megan E.
Myers, Leann
Intapad, Suttira
Lindsey, Sarah H.
Bayer, Carolyn L.
author_sort Lawrence, Dylan J.
collection PubMed
description Preeclampsia is a pregnancy-related hypertensive disorder accounting for 14% of global maternal deaths annually. Preeclampsia — maternal hypertension and proteinuria — is promoted by placental ischemia resulting from reduced uteroplacental perfusion. Here, we assess longitudinal changes in placental oxygenation during preeclampsia using spectral photoacoustic imaging. Spectral photoacoustic images were acquired of the placenta of normal pregnant (NP) and preeclamptic reduced uterine perfusion pressure (RUPP) Sprague Dawley rats on gestational days (GD) 14, 16, and 18, corresponding to mid- to late gestation (n = 10 per cohort). Two days after implementation of the RUPP surgical model, placental oxygen saturation decreased 12% in comparison with NP. Proteinuria was determined from a 24-hour urine collection prior to imaging on GD18. Blood pressure measurements were obtained on GD18 after imaging. Placental hypoxia in the RUPP was confirmed with histological staining for hypoxia-inducible factor (HIF)-1α, a cellular transcription regulator which responds to local oxygen levels. Using in vivo, longitudinal imaging methods we determined that the placenta in the reduced uterine perfusion pressure rat model of preeclampsia is hypoxic, and that this hypoxia is maintained through late gestation. Future work will utilize these methods to assess the impact of novel therapeutics on placental ischemia and the progression of preeclampsia.
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spelling pubmed-63459472019-01-29 Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia Lawrence, Dylan J. Escott, Megan E. Myers, Leann Intapad, Suttira Lindsey, Sarah H. Bayer, Carolyn L. Sci Rep Article Preeclampsia is a pregnancy-related hypertensive disorder accounting for 14% of global maternal deaths annually. Preeclampsia — maternal hypertension and proteinuria — is promoted by placental ischemia resulting from reduced uteroplacental perfusion. Here, we assess longitudinal changes in placental oxygenation during preeclampsia using spectral photoacoustic imaging. Spectral photoacoustic images were acquired of the placenta of normal pregnant (NP) and preeclamptic reduced uterine perfusion pressure (RUPP) Sprague Dawley rats on gestational days (GD) 14, 16, and 18, corresponding to mid- to late gestation (n = 10 per cohort). Two days after implementation of the RUPP surgical model, placental oxygen saturation decreased 12% in comparison with NP. Proteinuria was determined from a 24-hour urine collection prior to imaging on GD18. Blood pressure measurements were obtained on GD18 after imaging. Placental hypoxia in the RUPP was confirmed with histological staining for hypoxia-inducible factor (HIF)-1α, a cellular transcription regulator which responds to local oxygen levels. Using in vivo, longitudinal imaging methods we determined that the placenta in the reduced uterine perfusion pressure rat model of preeclampsia is hypoxic, and that this hypoxia is maintained through late gestation. Future work will utilize these methods to assess the impact of novel therapeutics on placental ischemia and the progression of preeclampsia. Nature Publishing Group UK 2019-01-24 /pmc/articles/PMC6345947/ /pubmed/30679723 http://dx.doi.org/10.1038/s41598-018-37310-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lawrence, Dylan J.
Escott, Megan E.
Myers, Leann
Intapad, Suttira
Lindsey, Sarah H.
Bayer, Carolyn L.
Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title_full Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title_fullStr Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title_full_unstemmed Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title_short Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
title_sort spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345947/
https://www.ncbi.nlm.nih.gov/pubmed/30679723
http://dx.doi.org/10.1038/s41598-018-37310-2
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