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Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems
Nematodes belong to one of the most diverse animal phyla. However, functional genomic studies in nematodes, other than in a few species, have often been limited in their reliability and success. Here we report that by combining liposome-based technology with microinjection, we were able to establish...
Autores principales: | Adams, Sally, Pathak, Prachi, Shao, Hongguang, Lok, James B., Pires-daSilva, Andre |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345965/ https://www.ncbi.nlm.nih.gov/pubmed/30679624 http://dx.doi.org/10.1038/s41598-018-37036-1 |
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