Cargando…
Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas
Plant populations growing along an altitudinal gradient are exposed to different environmental conditions. They are excellent resources to study regulatory mechanisms adopted by plants to respond to different environmental stresses. Regulation by miRNA is one of such strategies. Here, we report how...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345966/ https://www.ncbi.nlm.nih.gov/pubmed/30679759 http://dx.doi.org/10.1038/s41598-018-37465-y |
_version_ | 1783389668492967936 |
---|---|
author | Tripathi, Abhinandan Mani Singh, Akanksha Singh, Rajneesh Verma, Ashwani Kumar Roy, Sribash |
author_facet | Tripathi, Abhinandan Mani Singh, Akanksha Singh, Rajneesh Verma, Ashwani Kumar Roy, Sribash |
author_sort | Tripathi, Abhinandan Mani |
collection | PubMed |
description | Plant populations growing along an altitudinal gradient are exposed to different environmental conditions. They are excellent resources to study regulatory mechanisms adopted by plants to respond to different environmental stresses. Regulation by miRNA is one of such strategies. Here, we report how different miRNAs are preferentially expressed in the three natural populations of A. thaliana originating from a wide altitudinal range. The expression level of miRNAs was mostly governed by temperature and radiation. Majority of the identified miRNAs expressed commonly in the three populations. However, 30 miRNAs expressed significantly at different level between the low and the high altitude populations. Most of these miRNAs regulate the genes associated with different developmental processes, abiotic stresses including UV, cold, secondary metabolites, etc. Further, the expression of miR397 and miR858 involved in lignin biosynthesis and regulation of secondary metabolites respectively, may be regulated by light intensity. A few miRNAs expressed at increasing level with the increase in the altitude of the site indicating environment driven tight regulation of these miRNAs. Further, several novel miRNAs and isomiR diversity specific to the Himalayas are reported which might have an adaptive advantage. To the best of our knowledge, this is the first report on miRNA expression from natural plant populations. |
format | Online Article Text |
id | pubmed-6345966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63459662019-01-29 Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas Tripathi, Abhinandan Mani Singh, Akanksha Singh, Rajneesh Verma, Ashwani Kumar Roy, Sribash Sci Rep Article Plant populations growing along an altitudinal gradient are exposed to different environmental conditions. They are excellent resources to study regulatory mechanisms adopted by plants to respond to different environmental stresses. Regulation by miRNA is one of such strategies. Here, we report how different miRNAs are preferentially expressed in the three natural populations of A. thaliana originating from a wide altitudinal range. The expression level of miRNAs was mostly governed by temperature and radiation. Majority of the identified miRNAs expressed commonly in the three populations. However, 30 miRNAs expressed significantly at different level between the low and the high altitude populations. Most of these miRNAs regulate the genes associated with different developmental processes, abiotic stresses including UV, cold, secondary metabolites, etc. Further, the expression of miR397 and miR858 involved in lignin biosynthesis and regulation of secondary metabolites respectively, may be regulated by light intensity. A few miRNAs expressed at increasing level with the increase in the altitude of the site indicating environment driven tight regulation of these miRNAs. Further, several novel miRNAs and isomiR diversity specific to the Himalayas are reported which might have an adaptive advantage. To the best of our knowledge, this is the first report on miRNA expression from natural plant populations. Nature Publishing Group UK 2019-01-24 /pmc/articles/PMC6345966/ /pubmed/30679759 http://dx.doi.org/10.1038/s41598-018-37465-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Tripathi, Abhinandan Mani Singh, Akanksha Singh, Rajneesh Verma, Ashwani Kumar Roy, Sribash Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title | Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title_full | Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title_fullStr | Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title_full_unstemmed | Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title_short | Modulation of miRNA expression in natural populations of A. thaliana along a wide altitudinal gradient of Indian Himalayas |
title_sort | modulation of mirna expression in natural populations of a. thaliana along a wide altitudinal gradient of indian himalayas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345966/ https://www.ncbi.nlm.nih.gov/pubmed/30679759 http://dx.doi.org/10.1038/s41598-018-37465-y |
work_keys_str_mv | AT tripathiabhinandanmani modulationofmirnaexpressioninnaturalpopulationsofathalianaalongawidealtitudinalgradientofindianhimalayas AT singhakanksha modulationofmirnaexpressioninnaturalpopulationsofathalianaalongawidealtitudinalgradientofindianhimalayas AT singhrajneesh modulationofmirnaexpressioninnaturalpopulationsofathalianaalongawidealtitudinalgradientofindianhimalayas AT vermaashwanikumar modulationofmirnaexpressioninnaturalpopulationsofathalianaalongawidealtitudinalgradientofindianhimalayas AT roysribash modulationofmirnaexpressioninnaturalpopulationsofathalianaalongawidealtitudinalgradientofindianhimalayas |