Cargando…
Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial
Infant formulae have been used since decades as an alternative to or a complement to human milk. Human milk, the “gold standard” of infant nutrition, has been studied for its properties in order to create infant formulae that bring similar benefits to the infant. One of the characteristics of milk i...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346115/ https://www.ncbi.nlm.nih.gov/pubmed/30679671 http://dx.doi.org/10.1038/s41598-018-37210-5 |
_version_ | 1783389701034475520 |
---|---|
author | Smolinska, A. Baranska, A. Dallinga, J. W. Mensink, R. P. Baumgartner, S. van de Heijning, B. J. M. van Schooten, F. J. |
author_facet | Smolinska, A. Baranska, A. Dallinga, J. W. Mensink, R. P. Baumgartner, S. van de Heijning, B. J. M. van Schooten, F. J. |
author_sort | Smolinska, A. |
collection | PubMed |
description | Infant formulae have been used since decades as an alternative to or a complement to human milk. Human milk, the “gold standard” of infant nutrition, has been studied for its properties in order to create infant formulae that bring similar benefits to the infant. One of the characteristics of milk is the size of the lipid droplets which is known to affect the digestion, gastric emptying and triglyceride metabolism. In the current study a concept infant milk formula with large, phospholipid coating of lipid droplets (mode diameter 3–5 μm; NUTURIS, further described as “active”), was compared to a commercially available formula milk characterised by smaller lipid droplets, further described as “control” (both products derived from Nutricia). We investigated whether we could find an effect of lipid droplet size on volatile compounds in exhaled air upon ingestion of either product. For that purpose, exhaled breath was collected from a group of 29 healthy, non-smoking adult males before ingestion of a study product (baseline measurements, T0) and at the following time points after the test meal: 30, 60, 120, 180 and 240 min. Volatile organic compounds (VOCs) in breath were detected by gas chromatography-time-of-flight-mass spectrometry. Any differences in the time course of VOCs patterns upon intake of active and control products were investigated by regularised multivariate analysis of variance (rMANOVA). The rMANOVA analysis revealed statistically significant differences in the exhaled breath composition 240 min after ingestion of the active formula compared to control product (p-value < 0.0001), but did not show significant changes between active and control product at any earlier time points. A set of eight VOCs in exhaled breath had the highest contribution to the difference found at 240 minutes between the two formulas. A set of ten VOCs was different between baseline and the two formulae at T240 with p-value < 0.0001. To our knowledge this is the first study that shows the ability of VOCs in exhaled breath to monitor metabolic effects after ingestion of infant formulae with different lipid structure. The statistically significant differences in compound abundance found between active and control formula milk may be related to: (i) specific differences in the digestion, (ii) absorption of lipids and proteins and (iii) assimilation of the products in the gut. |
format | Online Article Text |
id | pubmed-6346115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-63461152019-01-29 Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial Smolinska, A. Baranska, A. Dallinga, J. W. Mensink, R. P. Baumgartner, S. van de Heijning, B. J. M. van Schooten, F. J. Sci Rep Article Infant formulae have been used since decades as an alternative to or a complement to human milk. Human milk, the “gold standard” of infant nutrition, has been studied for its properties in order to create infant formulae that bring similar benefits to the infant. One of the characteristics of milk is the size of the lipid droplets which is known to affect the digestion, gastric emptying and triglyceride metabolism. In the current study a concept infant milk formula with large, phospholipid coating of lipid droplets (mode diameter 3–5 μm; NUTURIS, further described as “active”), was compared to a commercially available formula milk characterised by smaller lipid droplets, further described as “control” (both products derived from Nutricia). We investigated whether we could find an effect of lipid droplet size on volatile compounds in exhaled air upon ingestion of either product. For that purpose, exhaled breath was collected from a group of 29 healthy, non-smoking adult males before ingestion of a study product (baseline measurements, T0) and at the following time points after the test meal: 30, 60, 120, 180 and 240 min. Volatile organic compounds (VOCs) in breath were detected by gas chromatography-time-of-flight-mass spectrometry. Any differences in the time course of VOCs patterns upon intake of active and control products were investigated by regularised multivariate analysis of variance (rMANOVA). The rMANOVA analysis revealed statistically significant differences in the exhaled breath composition 240 min after ingestion of the active formula compared to control product (p-value < 0.0001), but did not show significant changes between active and control product at any earlier time points. A set of eight VOCs in exhaled breath had the highest contribution to the difference found at 240 minutes between the two formulas. A set of ten VOCs was different between baseline and the two formulae at T240 with p-value < 0.0001. To our knowledge this is the first study that shows the ability of VOCs in exhaled breath to monitor metabolic effects after ingestion of infant formulae with different lipid structure. The statistically significant differences in compound abundance found between active and control formula milk may be related to: (i) specific differences in the digestion, (ii) absorption of lipids and proteins and (iii) assimilation of the products in the gut. Nature Publishing Group UK 2019-01-24 /pmc/articles/PMC6346115/ /pubmed/30679671 http://dx.doi.org/10.1038/s41598-018-37210-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Smolinska, A. Baranska, A. Dallinga, J. W. Mensink, R. P. Baumgartner, S. van de Heijning, B. J. M. van Schooten, F. J. Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title | Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title_full | Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title_fullStr | Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title_full_unstemmed | Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title_short | Comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
title_sort | comparing patterns of volatile organic compounds exhaled in breath after consumption of two infant formulae with a different lipid structure: a randomized trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346115/ https://www.ncbi.nlm.nih.gov/pubmed/30679671 http://dx.doi.org/10.1038/s41598-018-37210-5 |
work_keys_str_mv | AT smolinskaa comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT baranskaa comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT dallingajw comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT mensinkrp comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT baumgartners comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT vandeheijningbjm comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial AT vanschootenfj comparingpatternsofvolatileorganiccompoundsexhaledinbreathafterconsumptionoftwoinfantformulaewithadifferentlipidstructurearandomizedtrial |