Cargando…
Construction of supramolecular nanotubes from protein crystals
Investigations involving the design of protein assemblies for the development of biomaterials are receiving significant attention. In nature, proteins can be driven into assemblies frequently by various non-covalent interactions. Assembly of proteins into supramolecules can be conducted under limite...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346403/ https://www.ncbi.nlm.nih.gov/pubmed/30774900 http://dx.doi.org/10.1039/c8sc04167a |
_version_ | 1783389744327032832 |
---|---|
author | Nguyen, Tien Khanh Negishi, Hashiru Abe, Satoshi Ueno, Takafumi |
author_facet | Nguyen, Tien Khanh Negishi, Hashiru Abe, Satoshi Ueno, Takafumi |
author_sort | Nguyen, Tien Khanh |
collection | PubMed |
description | Investigations involving the design of protein assemblies for the development of biomaterials are receiving significant attention. In nature, proteins can be driven into assemblies frequently by various non-covalent interactions. Assembly of proteins into supramolecules can be conducted under limited conditions in solution. These factors force the assembly process into an equilibrium state with low stability. Here, we report a new method for preparing assemblies using protein crystals as non-equilibrium molecular scaffolds. Protein crystals provide an ideal environment with a highly ordered packing of subunits in which the supramolecular assembled structures are formed in the crystalline matrix. Based on this feature, we demonstrate the self-assembly of supramolecular nanotubes constructed from protein crystals triggered by co-oxidation with cross-linkers. The assembly of tubes is driven by the formation of disulfide bonds to retain the intermolecular interactions within each assembly in the crystalline matrix after dissolution of the crystals. |
format | Online Article Text |
id | pubmed-6346403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-63464032019-02-15 Construction of supramolecular nanotubes from protein crystals Nguyen, Tien Khanh Negishi, Hashiru Abe, Satoshi Ueno, Takafumi Chem Sci Chemistry Investigations involving the design of protein assemblies for the development of biomaterials are receiving significant attention. In nature, proteins can be driven into assemblies frequently by various non-covalent interactions. Assembly of proteins into supramolecules can be conducted under limited conditions in solution. These factors force the assembly process into an equilibrium state with low stability. Here, we report a new method for preparing assemblies using protein crystals as non-equilibrium molecular scaffolds. Protein crystals provide an ideal environment with a highly ordered packing of subunits in which the supramolecular assembled structures are formed in the crystalline matrix. Based on this feature, we demonstrate the self-assembly of supramolecular nanotubes constructed from protein crystals triggered by co-oxidation with cross-linkers. The assembly of tubes is driven by the formation of disulfide bonds to retain the intermolecular interactions within each assembly in the crystalline matrix after dissolution of the crystals. Royal Society of Chemistry 2018-10-30 /pmc/articles/PMC6346403/ /pubmed/30774900 http://dx.doi.org/10.1039/c8sc04167a Text en This journal is © The Royal Society of Chemistry 2019 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Nguyen, Tien Khanh Negishi, Hashiru Abe, Satoshi Ueno, Takafumi Construction of supramolecular nanotubes from protein crystals |
title | Construction of supramolecular nanotubes from protein crystals
|
title_full | Construction of supramolecular nanotubes from protein crystals
|
title_fullStr | Construction of supramolecular nanotubes from protein crystals
|
title_full_unstemmed | Construction of supramolecular nanotubes from protein crystals
|
title_short | Construction of supramolecular nanotubes from protein crystals
|
title_sort | construction of supramolecular nanotubes from protein crystals |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346403/ https://www.ncbi.nlm.nih.gov/pubmed/30774900 http://dx.doi.org/10.1039/c8sc04167a |
work_keys_str_mv | AT nguyentienkhanh constructionofsupramolecularnanotubesfromproteincrystals AT negishihashiru constructionofsupramolecularnanotubesfromproteincrystals AT abesatoshi constructionofsupramolecularnanotubesfromproteincrystals AT uenotakafumi constructionofsupramolecularnanotubesfromproteincrystals |