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Population genetic structure of Schistosoma bovis in Cameroon

BACKGROUND: Schistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present...

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Autores principales: Djuikwo-Teukeng, Félicité Flore, Kouam Simo, Alain, Allienne, Jean-François, Rey, Olivier, Njayou Ngapagna, Arouna, Tchuem-Tchuente, Louis Albert, Boissier, Jérôme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346511/
https://www.ncbi.nlm.nih.gov/pubmed/30678712
http://dx.doi.org/10.1186/s13071-019-3307-0
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author Djuikwo-Teukeng, Félicité Flore
Kouam Simo, Alain
Allienne, Jean-François
Rey, Olivier
Njayou Ngapagna, Arouna
Tchuem-Tchuente, Louis Albert
Boissier, Jérôme
author_facet Djuikwo-Teukeng, Félicité Flore
Kouam Simo, Alain
Allienne, Jean-François
Rey, Olivier
Njayou Ngapagna, Arouna
Tchuem-Tchuente, Louis Albert
Boissier, Jérôme
author_sort Djuikwo-Teukeng, Félicité Flore
collection PubMed
description BACKGROUND: Schistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present novel data on the population genetic structure of Schistosoma bovis, a highly widespread and prevalent schistosome infecting ruminants, and therefore of veterinary importance. METHODS: Adult S. bovis were sampled in the two main abattoirs of Cameroon (Yaoundé and Douala). Twenty-two cows originating from four distinct localities were sampled and a total of 218 parasites were recovered. All parasites were genotyped using a panel of 14 microsatellite markers and a sub-sample of 91 parasites were sequenced and characterized with the mitochondrial (cox1) and nuclear (ITS) genetic markers. RESULTS: No significant difference in allelic richness, heterozygosity, nucleotide diversity and haplotype diversity was observed between the populations. Additionally, no strong genetic structure was observed at the country scale. Our data also show that S. bovis is more polymorphic than its sister species, S. haematobium, and that the haplotype diversity is similar to that of S. mansoni while the nucleotide diversity does not significantly differ from that of S. haematobium. The resulting negative Tajima’s D* and Fu and Li’s D* indices could be a signature of population demographic expansion. No S. haematobium/S. bovis hybrids were observed in our populations, thus all samples were considered as pure S. bovis. CONCLUSIONS: This study provides novel insights into genetic diversity and population genetic structure of S. bovis. No strong genetic structure was observed at the country scale but some genetic indices could be associated as a signature of population demographic expansion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3307-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-63465112019-01-29 Population genetic structure of Schistosoma bovis in Cameroon Djuikwo-Teukeng, Félicité Flore Kouam Simo, Alain Allienne, Jean-François Rey, Olivier Njayou Ngapagna, Arouna Tchuem-Tchuente, Louis Albert Boissier, Jérôme Parasit Vectors Research BACKGROUND: Schistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present novel data on the population genetic structure of Schistosoma bovis, a highly widespread and prevalent schistosome infecting ruminants, and therefore of veterinary importance. METHODS: Adult S. bovis were sampled in the two main abattoirs of Cameroon (Yaoundé and Douala). Twenty-two cows originating from four distinct localities were sampled and a total of 218 parasites were recovered. All parasites were genotyped using a panel of 14 microsatellite markers and a sub-sample of 91 parasites were sequenced and characterized with the mitochondrial (cox1) and nuclear (ITS) genetic markers. RESULTS: No significant difference in allelic richness, heterozygosity, nucleotide diversity and haplotype diversity was observed between the populations. Additionally, no strong genetic structure was observed at the country scale. Our data also show that S. bovis is more polymorphic than its sister species, S. haematobium, and that the haplotype diversity is similar to that of S. mansoni while the nucleotide diversity does not significantly differ from that of S. haematobium. The resulting negative Tajima’s D* and Fu and Li’s D* indices could be a signature of population demographic expansion. No S. haematobium/S. bovis hybrids were observed in our populations, thus all samples were considered as pure S. bovis. CONCLUSIONS: This study provides novel insights into genetic diversity and population genetic structure of S. bovis. No strong genetic structure was observed at the country scale but some genetic indices could be associated as a signature of population demographic expansion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3307-0) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-24 /pmc/articles/PMC6346511/ /pubmed/30678712 http://dx.doi.org/10.1186/s13071-019-3307-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Djuikwo-Teukeng, Félicité Flore
Kouam Simo, Alain
Allienne, Jean-François
Rey, Olivier
Njayou Ngapagna, Arouna
Tchuem-Tchuente, Louis Albert
Boissier, Jérôme
Population genetic structure of Schistosoma bovis in Cameroon
title Population genetic structure of Schistosoma bovis in Cameroon
title_full Population genetic structure of Schistosoma bovis in Cameroon
title_fullStr Population genetic structure of Schistosoma bovis in Cameroon
title_full_unstemmed Population genetic structure of Schistosoma bovis in Cameroon
title_short Population genetic structure of Schistosoma bovis in Cameroon
title_sort population genetic structure of schistosoma bovis in cameroon
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346511/
https://www.ncbi.nlm.nih.gov/pubmed/30678712
http://dx.doi.org/10.1186/s13071-019-3307-0
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