Multiple-gene targeting and mismatch tolerance can confound analysis of genome-wide pooled CRISPR screens

BACKGROUND: Genome-wide loss-of-function screens using the CRISPR/Cas9 system allow the efficient discovery of cancer cell vulnerabilities. While several studies have focused on correcting for DNA cleavage toxicity biases associated with copy number alterations, the effects of sgRNAs co-targeting mu...

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Detalles Bibliográficos
Autores principales: Fortin, Jean-Philippe, Tan, Jenille, Gascoigne, Karen E., Haverty, Peter M., Forrest, William F., Costa, Michael R., Martin, Scott E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346559/
https://www.ncbi.nlm.nih.gov/pubmed/30683138
http://dx.doi.org/10.1186/s13059-019-1621-7

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