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Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle
GR24 is a synthetic strigolactone analog, demonstrated to regulate the development of plants and arbuscular mycorrhizal fungi. GR24 possesses anti-cancer and anti-apoptotic properties, enhances insulin sensitivity and mitochondrial biogenesis in skeletal myotubes, inhibits adipogenesis, decreases in...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347031/ https://www.ncbi.nlm.nih.gov/pubmed/30728949 http://dx.doi.org/10.12688/f1000research.16172.2 |
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author | Modi, Shalem Raju Kokkola, Tarja |
author_facet | Modi, Shalem Raju Kokkola, Tarja |
author_sort | Modi, Shalem Raju |
collection | PubMed |
description | GR24 is a synthetic strigolactone analog, demonstrated to regulate the development of plants and arbuscular mycorrhizal fungi. GR24 possesses anti-cancer and anti-apoptotic properties, enhances insulin sensitivity and mitochondrial biogenesis in skeletal myotubes, inhibits adipogenesis, decreases inflammation in adipocytes and macrophages and downregulates the expression of hepatic gluconeogenic enzymes. Transcription factor Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) is a master regulator of antioxidant response, regulating a multitude of genes involved in cellular stress responses and anti-inflammatory pathways, thus maintaining cellular redox homeostasis. Nrf2 activation reduces the deleterious effects of mitochondrial toxins and has multiple roles in promoting mitochondrial function and dynamics. We studied the role of GR24 on gene expression in rat L6 skeletal muscle cells which were differentiated into myotubes. The myotubes were treated with GR24 and analyzed by microarray gene expression profiling. GR24 upregulated the cytoprotective transcription factor Nrf2 and its target genes, activating antioxidant defences, suggesting that GR24 may protect skeletal muscle from the toxic effects of oxidative stress. |
format | Online Article Text |
id | pubmed-6347031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-63470312019-02-05 Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle Modi, Shalem Raju Kokkola, Tarja F1000Res Research Note GR24 is a synthetic strigolactone analog, demonstrated to regulate the development of plants and arbuscular mycorrhizal fungi. GR24 possesses anti-cancer and anti-apoptotic properties, enhances insulin sensitivity and mitochondrial biogenesis in skeletal myotubes, inhibits adipogenesis, decreases inflammation in adipocytes and macrophages and downregulates the expression of hepatic gluconeogenic enzymes. Transcription factor Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) is a master regulator of antioxidant response, regulating a multitude of genes involved in cellular stress responses and anti-inflammatory pathways, thus maintaining cellular redox homeostasis. Nrf2 activation reduces the deleterious effects of mitochondrial toxins and has multiple roles in promoting mitochondrial function and dynamics. We studied the role of GR24 on gene expression in rat L6 skeletal muscle cells which were differentiated into myotubes. The myotubes were treated with GR24 and analyzed by microarray gene expression profiling. GR24 upregulated the cytoprotective transcription factor Nrf2 and its target genes, activating antioxidant defences, suggesting that GR24 may protect skeletal muscle from the toxic effects of oxidative stress. F1000 Research Limited 2018-11-14 /pmc/articles/PMC6347031/ /pubmed/30728949 http://dx.doi.org/10.12688/f1000research.16172.2 Text en Copyright: © 2018 Modi SR and Kokkola T http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Note Modi, Shalem Raju Kokkola, Tarja Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title | Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title_full | Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title_fullStr | Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title_full_unstemmed | Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title_short | Strigolactone GR24 upregulates target genes of the cytoprotective transcription factor Nrf2 in skeletal muscle |
title_sort | strigolactone gr24 upregulates target genes of the cytoprotective transcription factor nrf2 in skeletal muscle |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347031/ https://www.ncbi.nlm.nih.gov/pubmed/30728949 http://dx.doi.org/10.12688/f1000research.16172.2 |
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