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A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species
Perception of inclination in the gravity field and perception of light direction are two important environmental signals implicated in the control of plant shape and habit. However, their quantitative study in light-grown plants remains a challenge. We present a novel method here to determine the se...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347157/ https://www.ncbi.nlm.nih.gov/pubmed/30682051 http://dx.doi.org/10.1371/journal.pone.0209973 |
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author | Coutand, Catherine Adam, Boris Ploquin, Stéphane Moulia, Bruno |
author_facet | Coutand, Catherine Adam, Boris Ploquin, Stéphane Moulia, Bruno |
author_sort | Coutand, Catherine |
collection | PubMed |
description | Perception of inclination in the gravity field and perception of light direction are two important environmental signals implicated in the control of plant shape and habit. However, their quantitative study in light-grown plants remains a challenge. We present a novel method here to determine the sensitivities to gravitropism and phototropism. The method combines: (i) an original experimental device of isotropic light to disentangle gravitropic and phototropic plant responses; and (ii) model-assisted phenotyping using recent models of tropism perception—the AC model for gravitropism alone and the ArC model for gravitropism combined with phototropism. We first assessed the validity of the AC and ArC models on poplar, the classical species model for woody plants. We then tested the method on three woody species contrasted by their habit and tolerance to shade: poplar (Populus tremula*alba), oak (Quercus petraea) and beech (Fagus sylvatica). The method was found to be effective to quantitatively discriminate the tested species by their ratio of tropistic sensitivities. The method thus appears as an interesting tool to quantitatively determine tropistic sensitivities, a prerequisite for assessing the role of tropisms in the control of the variability of the habit and/or tolerance to shade of woody species in the future. |
format | Online Article Text |
id | pubmed-6347157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-63471572019-02-02 A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species Coutand, Catherine Adam, Boris Ploquin, Stéphane Moulia, Bruno PLoS One Research Article Perception of inclination in the gravity field and perception of light direction are two important environmental signals implicated in the control of plant shape and habit. However, their quantitative study in light-grown plants remains a challenge. We present a novel method here to determine the sensitivities to gravitropism and phototropism. The method combines: (i) an original experimental device of isotropic light to disentangle gravitropic and phototropic plant responses; and (ii) model-assisted phenotyping using recent models of tropism perception—the AC model for gravitropism alone and the ArC model for gravitropism combined with phototropism. We first assessed the validity of the AC and ArC models on poplar, the classical species model for woody plants. We then tested the method on three woody species contrasted by their habit and tolerance to shade: poplar (Populus tremula*alba), oak (Quercus petraea) and beech (Fagus sylvatica). The method was found to be effective to quantitatively discriminate the tested species by their ratio of tropistic sensitivities. The method thus appears as an interesting tool to quantitatively determine tropistic sensitivities, a prerequisite for assessing the role of tropisms in the control of the variability of the habit and/or tolerance to shade of woody species in the future. Public Library of Science 2019-01-25 /pmc/articles/PMC6347157/ /pubmed/30682051 http://dx.doi.org/10.1371/journal.pone.0209973 Text en © 2019 Coutand et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Coutand, Catherine Adam, Boris Ploquin, Stéphane Moulia, Bruno A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title | A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title_full | A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title_fullStr | A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title_full_unstemmed | A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title_short | A method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: Exploratory test of the method on three hardwood tree species |
title_sort | method for the quantification of phototropic and gravitropic sensitivities of plants combining an original experimental device with model-assisted phenotyping: exploratory test of the method on three hardwood tree species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347157/ https://www.ncbi.nlm.nih.gov/pubmed/30682051 http://dx.doi.org/10.1371/journal.pone.0209973 |
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