Protein refolding based on high hydrostatic pressure and alkaline pH: Application on a recombinant dengue virus NS1 protein

In this study we evaluated the association of high hydrostatic pressure (HHP) and alkaline pH as a minimally denaturing condition for the solubilization of inclusion bodies (IBs) generated by recombinant proteins expressed by Escherichia coli strains. The method was successfully applied to a recombi...

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Detalles Bibliográficos
Autores principales: Chura-Chambi, Rosa Maria, da Silva, Cleide Mara Rosa, Pereira, Lennon Ramos, Bartolini, Paolo, Ferreira, Luis Carlos de Souza, Morganti, Ligia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347194/
https://www.ncbi.nlm.nih.gov/pubmed/30682103
http://dx.doi.org/10.1371/journal.pone.0211162

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6347194/
https://www.ncbi.nlm.nih.gov/pubmed/30682103
http://dx.doi.org/10.1371/journal.pone.0211162

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